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|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|Clinical Chemistry||Plasma,Serum||CHOLESTEROL||Tests per Package: 1369|
|Pchem Reagents||The Pchem Cholesterol Reagent Kit has been designed for use in the quantitative determination in vitro of the Cholesterol in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.||Code: ADA-R0100000501||Package: R1 6 x 48.5 mL|
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The Pchem Cholesterol Reagent Kit has been designed for use in the quantitative determination in vitro of the Cholesterol in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.
This kit is intended for In vitro diagnostic use only, must not be sold to the general public and the test has to be carried out by a health-care professional person.
The kit contains reagents sufficient for 1369 tests.
Cholesterol is an essential component of the cell membrane of all animal cells. Cholesterol forms the basis for the synthesis of steroid hormones such as aldosterone, cortisone and testosterone; it is also essential for development of the embryo. Although part of the cholesterol present in the human body is derived from the diet, it is mostly produced in the liver and used largely for bile production. As cholesterol, like all lipids, is insoluble in the blood, aggregated complexes such as lipoproteins are used for its transportation in circulation. For this reason, when in medicine the term “cholesterol” is used, reference is in fact being made to lipoproteins which circulate in the blood, the concentration of which is known as cholesterolemia.
Esterified cholesterol is hydrolyzed into free cholesterol and fatty acid by cholesterol esterase (CE). Cholesterol oxidase (CO) then oxidates the free cholesterol to cholest-4-en-3-one with formation of hydrogen peroxide which, in the presence of peroxidise (POD), reacts with hydroxybenzoate (HBA and 4-aminophenazone, giving rise to a coloured compound, the intensity of which is read at a wavelength of 510 nm; the result is directly proportional to the cholesterol concentration in the sample.
Pchem Cholesterol Reagent Kit
Reagent (R) – 6 x 48.5 mL/vial