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|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|Clinical Chemistry||Serum||IRON TIBC||Tests per Package: 400|
|Pchem Reagents||The Pchem Iron TIBC Reagent Kit has been designed for the quantitative in vitro determination of the transferrine in human serum, to be interpreted in clinical contexts.||Code: ADA-R0200000600||Package: R1 4 x 45 mL; R2 2 x 18 mL; R3 2 x 18 mL|
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The Pchem Iron TIBC Reagent Kit has been designed for the quantitative in vitro determination of the transferrine in human serum, to be interpreted in clinical contexts.
This kit is intended for In vitro diagnostic use only, must not be sold to the general public and the test has to be carried out by a health-care professional person.
The kit contains reagents sufficient for 400 tests.
In blood, iron is binding to transferrine, a protein involved in electrophoretic migration with the beta-globulines. Transferrine is measured as the quantity of iron that the protein is able to bind (Total Iron Binding Capacity o TIBC). The quantity of iron that transferrine is able to binding other the iron already bond determinate the Insatured Iron-Binding Capacity UIBC. TIBC is the addition of UIBC and serum iron (in physiological conditions corrisponds to 250-425 µg/dL). The level of insaturated transferrine increases in sideropenic anemia and decreases in hemolytic anemia, ineffective erytropoiesis and in ipoproteins diseases.
Under alkaline enviroment free transferrine present in the sample is saturated by iron (Fe3+), following the elimination of iron (Fe3+) unbound , presents in excess.Under acid enviroment iron is libarated from transferrine and then after riduction at ferrous state (Fe2+), iron reacts with Ferene S to form a stable coloured complex with a maximum of absorbance at 600nm.
The intensity of the colour measured is proportional at the quantity of iron presents in the sample.
Pchem Iron TIBC Reagent Kit
Reagent (R1) - 4 x 45 mL/vial
Reagent (R2) - 2 x 18 mL/vial
Reagent (R3) - 2 x 18 mL/vial