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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Serum||EIAgen Thyroglobulin Kit||Tests per Package: 96|
|EIAgen||The EIAgen Thyroglobulin Kit has been designed for the quantitative determination of Thyroglobulin (Tg) Concentration in Human Serum.||Code: 11TG-100F1||Package: 1 Microplate|
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The EIAgen Thyroglobulin Kit has been designed for the quantitative determination of Thyroglobulin (Tg) Concentration in Human Serum.
Human thyroglobulin (Tg) is a large glycoprotein (660 kD) that is stored in the follicular colloid of the thyroid gland. It functions as a prohormone in the intrathyroid synthesis of primary thyroid hormones like Triiodothyronine (T3) and Thyroxine (T4).
Tg is elevated in thyroid follicular and papillary carcinoma, thyroid adenoma, subacute thyroiditis, Hashimoto’s thyroiditis and Graves Disease. Tg levels are found to be normal in patients with medullary thyroid carcinoma. Serial measurements of Tg is most useful in detecting recurrence of differentiated thyroid carcinoma following surgical resection or radioactive iodine ablation. Tg determination is used as an adjunct to iodine scanning but not as a replacement for it. Assessment of Tg levels aids in management of infants with congenital hypothyroidism.
Tg determination has been done with various methods using direct competitive binding RIA and double antibody sandwich IRMA or Elisa, of which latter is more useful. All these methods suffer from interference by endogenous autoantibodies to Tg. It is useful to determine the effect of autoantibodies before screening such patients for levels of Tg. Adaltis provides Tg autoanibody Elisa to rule out such interference.
The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (enzyme and immobilized), with different and distinct epitope recognition, in excess, and native antigen. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal Thyroglobulinantibody.
Upon mixing monoclonal biotinylated antibody, and a serum containing the native antigen, reaction results between the native antigen and the antibody, forming an antibody-antigen complex. Simultaneously the biotin attached to the antibody binds to the streptavidin coated on the microwells resulting in immobilization of the complex.
After a suitable incubation period, the antibody-antigen bound fraction is separated from unbound antigen by decantation or aspiration. Another antibody (directed at a different epitope) labeled with an enzyme is added. Another interaction occurs to form an enzyme labeled antibody-antigen-biotinylated-antibody complex on the surface of the wells. Excess enzyme is washed off via a wash step. A suitable substrate is added to produce color measurable with the use of a microplate spectrophotometer. The enzyme activity on the well is directly proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code 11TG-100F1).
Bring reagents to room temperature before use.
Wash Buffer Conc. 50X
Number of tests