IVD SYSTEMS & AUTOMATED CLINICAL DIAGNOSTIC ANALYSERS
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MicroELISA

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Method & Line Sample & Target Product Package Info Product Package Info
MicroELISA Plasma,Serum EIAgen Anti-HBsAg Kit Tests per Package: 96
EIAgen Enzyme ImmunoAssay (ELISA) for both the quantitative and qualitative determination of antibodies to Hepatitis B Surface Antigen in human plasma and sera. For “in vitro” diagnostic use only. Code: 071001 Package: 1 Microplate
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  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

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Enzyme ImmunoAssay (ELISA) for both the quantitative and qualitative determination of antibodies to Hepatitis B Surface Antigen in human plasma and sera. For “in vitro” diagnostic use only.

The World Health Organization (WHO) defines Hepatitis B Virus infection as follows:

“Hepatitis B is one of the major diseases of mankind and is a serious global public health problem. Hepatitis means inflammation of the liver, and the most common cause is infection with one of 5 viruses, called hepatitis A,B,C,D, and E. All of these viruses can cause an acute disease with symptoms lasting several weeks including yellowing of the skin and eyes (jaundice); dark urine; extreme fatigue; nausea; vomiting and abdominal pain. It can take several months to a year to feel fit again. Hepatitis B virus can cause chronic infection in which the patient never gets rid of the virus and many years later develops cirrhosis of the liver or liver cancer. HBV is the most serious type of viral hepatitis and the only type causing chronic hepatitis for which a vaccine is available. Hepatitis B virus is transmitted by contact with blood or body fluids of an infected person in the same way as human immunodeficiency virus (HIV), the virus that causes AIDS. However, HBV is 50 to 100 times more infectious than HIV. The main ways of getting infected with HBV are: (a) perinatal (from mother to baby at the birth); (b) child- to child transmission; (c) unsafe injections and transfusions; (d) sexual contact. Worldwide, most infections occur from infected mother to child, from child to child contact in household settings, and from reuse of un-sterilized needles and syringes. In many developing countries, almost all children become infected with the virus. In many industrialized countries (e.g. Western Europe and North America), the pattern of transmission is different. In these countries, mother-to-infant and child-to-child transmission accounted for up to one third of chronic infections before childhood hepatitis B vaccination programmes were implemented. However, the majority of infections in these countries are acquired during young adulthood by sexual activity, and injecting drug use. In addition, hepatitis B virus is the major infectious occupational hazard of health workers, and most health care workers have received hepatitis B vaccine. Hepatitis B virus is not spread by contaminated food or water, and cannot be spread casually in the workplace. High rates of chronic HBV infection are also found in the southern parts of Eastern and Central Europe. In the Middle East and Indian sub-continent, about 5% are chronically infected. Infection is less common in Western Europe and North America, where less than 1% are chronically infected. Young children who become infected with HBV are the most likely to develop chronic infection. About 90% of infants infected during the first year of life and 30% to 50% of children infected between 1 to 4 years of age develop chronic infection. The risk of death from HBV-related liver cancer or cirrhosis is approximately 25% for persons who become chronically infected during childhood. Chronic hepatitis B in some patients is treated with drugs called interferon or lamivudine, which can help some patients. Patients with cirrhosis are sometimes given liver transplants, with varying success. It is preferable to prevent this disease with vaccine than to try and cure it. Hepatitis B vaccine has an outstanding record of safety and effectiveness. Since 1982, over one billion doses of hepatitis B vaccine have been used worldwide. The vaccine is given as a series of three intramuscular doses. Studies have shown that the vaccine is 95% effective in preventing children and adults from developing chronic infection if they have not yet been infected. In many countries where 8% to 15% of children used to become chronically infected with HBV, the rate of chronic infection has been reduced to less than 1% in immunized groups of children. Since 1991, WHO has called for all countries to add hepatitis B vaccine into their national immunization programmes.” Hepatitis B surface Antigen (HBsAg) is the major structural polypeptide of the envelope of the Hepatitis B Virus (HBV). This antigen is composed mainly of the type common determinant “a” and the type specific determinants “d” and “y”, present only on the specific serotypes. Upon infection, a strong immunological response develops firstly against the type specific determinants and in a second time against the “a” determinant. Anti “a” antibodies are however recognised to be most effective in the neutralisation of the virus, protecting the patient from other infections and leading it to convalescence. The detection of HBsAb has become important for the follow up of patients infected by HBV and the monitoring of recipients upon vaccination with synthetic and natural HBsAg.

Microplates are coated with a preparation of highly purified HBsAg that in the first incubation with sample specifically captures anti HBsAg antibodies to the solid phase.

After washing, captured antibodies are detected by an HBsAg, labelled with peroxidase (HRP), that specifically binds the second available binding site of these antibodies.

The enzyme specifically bound to wells, by acting on the substrate TMB mixture, generates an optical signal that is proportional to the amount of HBsAb in the sample and can be detected by an ELISA reader.

The amount of antibodies may be quantitated by means of a standard curve calibrated against the W.H.O reference preparation.

Samples are pre treated in the well with a specimen diluent able to block interference present in vaccinated individuals.

Each kit contains sufficient reagents to perform 96 tests (code 071001).

Microplate

Calibrators

Control Serum

Wash Buffer Concentrate 20x

Conjugate

Substrate TMB

Stop Solution

Sample Diluent

Plate seal foils

Package insert

1

5x2.0 mL/vials

2 vials

1x60 mL/vial

1x16 mL/vial

1x16 mL/vial

1x15 mL/vial

1x8 mL/vial

2

1

Number of tests

96

Code

071001

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