MicroELISA

Please pay attention to the revision of the document that must be the same as the revision reported in the box label.
In case of discrepancy please contact our Customer Care e-mail: info@adaltis.net.

* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.

Enzyme ImmunoAssay (ELISA) for   the   determination of   IgM class antibodies to Hepatitis Delta Virus or HDV in human plasma and sera with the "capture" system. The kit is intended for the classification of the viral infective agent and the follow-up of HDV infected patients. For “in vitro” diagnostic use only.

The Hepatitis Delta Virus or HDV is a RNA defective virus composed of a core presenting the delta-specific antigen, encapsulated by HBsAg, that requires the helper function of HBV to support its replication.

Infection by HDV occurs in the presence of acute or chronic HBV infection. When acute delta and acute HBV simultaneously occur, the illness becomes severe and clinical and biochemical features may be indistinguishable from those of HBV infection alone. In contrast, a patient with chronic HBV infection can support HDV replication indefinitely, usually with a less severe illness appearing as a clinical exacerbation.

The determination of HDV specific serological markers (HDV Ag, HDV IgM and HDV IgG) represents in these cases an important tool to the clinician for the classification of the etiological agent, for the follow up of infected patients and their treatment. The detection of HDV IgM and IgG antibodies allows the classification of the illness and the monitoring of the seroconversion event.

Microplates are coated with a monoclonal anti-hIgM antibody that in the 1^st incubation “captures” specifically this class of antibodies.

After washing out all the other components of the sample, in the 2^nd incubation bound anti HDV IgM are detected by the addition of recombinant HDV antigen immunocomplexed with a specific antibody, labeled with peroxidase (HRP).

After washing, the enzyme captured on the solid phase, acting on the substrate TMB mixture, generates an optical signal that is proportional to the amount of IgM antibodies present in the sample.

Each kit contains sufficient reagents to carry out 96 tests.