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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Plasma,Serum||EIAgen 17β-Estradiol Kit||Tests per Package: 96|
|EIAgen||The EIAgen 17β-Estradiol Kit has been designed for the quantitative determination of Estradiol Concentration in Human Serum or Plasma.||Code: LI4041F1||Package: 1 Microplate|
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The EIAgen 17β-Estradiol Kit has been designed for the quantitative determination of Estradiol Concentration in Human Serum or Plasma.
Measurement of estradiol in serum or plasma is considered to be the most reliable way to assess its rate of production. Estradiol (17β-estradiol) is a steroid hormone (molecular weight of 272.3 daltons), which circulates predominantly protein-bound. In addition to estradiol, other natural steroidal estrogens include estrone, estriol and their metabolites. Natural estrogens are hormones secreted principally by the ovarian follicles and also by the adrenals, corpus luteum, and placenta and, in males, by the testes. Exogenous estrogens (natural or synthetic) elicit, to varying degrees, all the pharmacologic responses usually produced by endogenous estrogens. Estrogenic hormones are secreted at varying rates during the menstrual cycle throughout the period of ovarian activity. During pregnancy, the placenta becomes the main source of estrogens. At menopause, ovarian secretion of estrogens declines at varying rates. The gonadotropins of the anterior pituitary regulate secretion of the ovarian hormones, estradiol and progesterone; hypothalamic control of pituitary gonadotropin production is in turn regulated by plasma concentrations of the estrogens and progesterone. This complex feedback system results in the cyclic phenomenon of ovulation and menstruation. Estradiol determinations have proved of value in a variety of contexts, including the investigation of precocious puberty in girls and gynecomastia in men. Its principal uses have been in the differential diagnosis of amenorrhea and in the monitoring of ovulation induction. This kit uses a specific anti-estradiol antibody, and does not require prior sample extraction of serum or plasma. Cross-reactivity to other naturally occurring and structurally related steroids is low. The employment of several serum references of known estradiol concentration permits construction of a graph of activity and concentration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with estradiol concentration.
The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing the biotinylated antibody with a serum containing the antigen, a reaction results between the antigen and the antibody. The interaction is illustrated by the following equation:
After a short incubation, the enzyme conjugate is added (This delayed addition permits an increase in sensitivity for low concentration samples). Upon the addition of the enzyme conjugate, competition reaction results between the enzyme analog and the antigen in the sample for a limited number of antibody binging sites (not consumed in the first incubation).
A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration.
The enzyme activity in the antibody bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen con- centration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI4041F1).
Bring reagents to room temperature before use.
Wash Buffer Conc. 50X
Number of tests