- Home Page
- Sales & Partners
- News & Events
- Site Map
|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|MicroELISA||Plasma,Serum||EIAgen Cortisol Kit||Tests per Package: 96|
|EIAgen||The EIAgen Cortisol Kit has been designed for the quantitative determination of Cortisol Concentration in Human Serum or Plasma.||Code: LI4003KF1||Package: 1 Microplate|
Please pay attention to the revision of the document that must be the same as the revision reported in the box label.
In case of discrepancy please contact our Customer Care e-mail: firstname.lastname@example.org.
* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.
The EIAgen Cortisol Kit has been designed for the quantitative determination of Cortisol Concentration in Human Serum or Plasma.
Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid produced by the human adrenal cortex. As with other adrenal steroids, cortisol is synthesized from cholesterol, through a series of enzymatically mediated steps, by the adrenal cortex [reviewed in 1, 2]. The first and rate-limiting step in adrenal steroidogenesis, conversion of cholesterol to pregnenolone, is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is, in turn, regulated by hypothalamic corticotropin releasing factor (CRF). ACTH and CRF secretion are inhibited by high cortisol levels. In plasma, the major portion of cortisol is bound with high affinity to corticosteroid-binding globulin (CBG, transcortin), with most of the remainder loosely bound to albumin. Physiologically effective in anti-inflammatory activity and blood pressure maintenance, cortisol is also involved in gluconeogenesis. Cortisol acts through specific intracellular receptors and has effects in numerous other physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism [1-3]. Cortisol is excreted primarily in urine in an unbound (free) form.
Cortisol production has an ACTH-dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadian rhythm are not completely defined. The circadian rhythm of ACTH/cortisol secretion matures gradually during early infancy, and is disrupted in a number of physical and psychological conditions . Furthermore, increased amounts of ACTH and cortisol are secreted independently of the circadian rhythm in response to physical and psychological stress [4, 5].
Elevated cortisol levels and lack of diurnal variation have been identified in patients with Cushing's disease (ACTH hyper secretion) [2, 6]. Elevated circulating cortisol levels have also been identified in patients with adrenal tumors . Low cortisol levels are found in primary adrenal insufficiency (e.g. adrenal hypoplasia, congenital adrenal hyperplasia, Addison's disease) and in ACTH deficiency [1, 2, 8, and 9]. Due to the normal circadian variation of cortisol levels, distinguishingnormal and abnormally low cortisol levels can be difficult. Therefore, various tests to evaluate the pituitary-adrenal (ACTH-cortisol) axis,including insulin-induced hypoglycemia, short- and long-term ACTH stimulation, CRF stimulation and artificial blockage of cortisol synthesis with metronome have been performed [8-10]. Cortisol response characteristics for each of these procedures have been reported.
The EIAgen Cortisol Kit uses a specific monoclonal anti-cortisol antibody, and does not require prior sample extraction of serum or plasma. Cross-reactivity to other naturally-occurring steroids is low.
The employment of several serum references of known cortisol concentration permits construction of a graph of activity and concentration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with cortisol concentration.
The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen.
Upon mixing biotinylated antibody, enzyme-antigen conjugate and a serum containing the native antigen, a competition reaction results between the native antigen and the enzyme-antigen conjugate for a limited number of antibody binding sites.
A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration.
The enzyme activity in the antibody‑bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI4003KF1).
Bring reagents to room temperature before use.
Conjugate Conc. 11X
Wash Buffer Conc. 50X
Number of tests