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|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|MicroELISA||Serum,Urine||EIAgen DHEA-S Kit||Tests per Package: 96|
|EIAgen||The EIAgen DHEA-S Kit has been designed for the quantitative determination of Dehydroepiandrosterone Sulfate (DHEA-S) Concentration in Human Serum or Plasma.||Code: LI4004KF1||Package: 1 Microplate|
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The EIAgen DHEA-S Kit has been designed for the quantitative determination of Dehydroepiandrosterone Sulfate (DHEA-S) Concentration in Human Serum or Plasma.
Dehydroepiandrosterone sulfate (DHEA-S) is the major C19 steroid secreted by the adrenal cortex, and is a precursor in testosterone and estrogen biosynthesis. DHEA-S, the sulfate ester of DHEA, is derived from sulfated precursors and by enzymatic conversion of DHEA in adrenal and extradrenal tissues. Due to the presence of a 17-oxo [rather than hydroxyl] group, DHEA-S possesses relatively weak androgenic activity, which for unsulfated DHEA has been estimated at ~10% that of testosterone . However, the bioactivity of DHEA-S may be increased by its relatively high serum concentrations, approximately 100 to 1000-fold higher than DHEA or testosterone, and its weak affinity for sex-hormone binding globulin . The physiologic role of DHEA-S is not well-defined. Serum levels are relatively high in the fetus and neonate, low during childhood, and increase during puberty [3, 4]. Increased levels of DHEA-S during adrenarche may contribute to the development of secondary sexual hair. DHEA-S levels show a progressive decline after the third decade of life . Unlike DHEA, DHEA-S levels do not show significant diurnal variation, show little day-to-day variation, are not responsive to acute corticotropin administration , and do not vary significantly during the normal menstrual cycle . This may be due to the slower metabolic clearance rate of DHEA-S as compared to DHEA . Measurement of serum DHEA-S is a useful marker of adrenal androgen synthesis. Abnormally low levels have been reported in hypoadrenalism , while elevated levels occur in several conditions; including virilizing adrenal adenoma and carcinoma , 21-hydroxylase and 3β-hydroxysteroid dehydrogenase deficiencies [2,6] and some cases of female hirsutism . Since very little DHEA-S is produced by the gonads [2, 3], measurement of DHEA-S may aid in the localization of the androgen source in virilizing conditions. Methods for measurement of DHEA-S include gas-liquid chromatography, double-isotope derivative techniques, competitive protein-binding assays, and radioimmunoassay. Although significant cross-reactivity occurs with DHEA, androstenedione and androsterone, the relative concentrations of these competing substances in most normal and pathologic samples predicts a minimal effect on assay performance.
The EIAgen DHEA-S Kit uses a specific anti-DHEA-S antibody, and does not require prior sample extraction of serum or plasma. Cross-reactivity to other naturally occurring and structurally related steroids is low.
The employment of several serum references of known DHEA-S concentration permits construction of a graph of activity and concentration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with DHEA-S concentration.
The essential reagents required for an enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen.
Upon mixing biotinylated antibody, enzyme-antigen conjugate and a serum containing the native antigen, a competition reaction results between the native antigen and the enzyme-antigen conjugate for a limited number of antibody binding sites.
A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration.
The enzyme activity in the antibody bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI4004KF1).
Bring reagents to room temperature before use.
Wash Buffer Conc. 50X
Number of tests