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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Plasma,Serum||EIAgen Progesterone (C21) Kit||Tests per Package: 96|
|EIAgen||The EIAgen Progesterone (C21) Kit has been designed for the quantitative determination of Progesterone Concentration in Human Serum or Plasma.||Code: LI4043F1||Package: 1 Microplate|
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The EIAgen Progesterone (C21) Kit has been designed for the quantitative determination of Progesterone Concentration in Human Serum or Plasma.
Measurement of progesterone in serum or plasma is considered to be the most reliable way to assess its rate of production.
Progesterone is a steroid hormone, which plays an important role in the preparation for and maintenance of pregnancy. It is synthesized from cholesterol via pregnenolone - then rapidly metabolized to pregnanediol primarily in the liver.2, 9,13 The ovary and placenta are the major production sites; but a small amount is also produced by the adrenal cortex in both men and women. Circulating progesterone levels, which are characteristically low during the follicular phase, increase sharply during the luteal phase of menstrual cycles, reaching a maximum approximately 5 to 10 days after the midcycle LH peak.12 Unless pregnancy occurs, a steep decline to follicular levels sets in about 4 days before the next menstrual period. This pattern constitutes the rationale behind the well established use of serum progesterone measurements as a simple and reliable method for ovulation detection.3,4,16
For routine measurements, immunoassays using steroid specific antibodies are preferred. Initial immunoassays, for serum progesterone, used organic solvents to remove the steroid from endogenous binding proteins such as corticosteroid binding globulin (CBG) and albumin. Direct measurement of progesterone in serum or plasma is considered to be the method of choice for routine applications. Both RIA and EIA (and some FIA) are available in the market. Since RIA involves handling radioactivity and causes radioactive waste disposal issues, various non-isotopic methods have replaced the RIA. These methods use very specific antibodies to determine levels of progesterone in circulation.
The EIAgen Progesterone (C21) Kit uses a specific anti-progesterone antibody, and does not require sample extraction of serum or plasma. Cross-reactivity to other naturally occurring and structurally related steroids is low.
The employment of several serum references of known progesterone concentration permits construction of a graph of activity and concentration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with progesterone concentration.
The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing the biotinylated antibody with a serum containing the antigen, a reaction results between the antigen and the antibody.
A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration.
The enzyme activity in the antibody bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI4043F1).
Bring reagents to room temperature before use.
Wash Buffer Conc. 50X
Number of tests