IVD SYSTEMS & AUTOMATED CLINICAL DIAGNOSTIC ANALYSERS
banner35.jpg

MicroELISA

All Reagents You Need, Available in One Click

Application Product Information & Purchase Order

Method & Line Sample & Target Product Package Info Product Package Info
MicroELISA Serum EIAgen HGH Kit Tests per Package: 96
EIAgen EIAgen hGH kit is a direct solid phase enzyme immunoassay for the quantitative measurement of Growth Hormone in human serum. Code: LI4016 Package: 1 Microplate
image not exist
  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

Please pay attention to the revision of the document that must be the same as the revision reported in the box label.
In case of discrepancy please contact our Customer Care e-mail: info@adaltis.net.

* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.

EIAgen hGH kit is a direct solid phase enzyme immunoassay for the quantitative measurement of Growth Hormone in human serum.

Growth hormone (hGH, somatotropin), secreted from the anterior pituitary, is a polypeptide with two intra-chain disulfide bridges, which circulates free or bound to number of different HGH-binding proteins. Several forms of growth hormone have been identified (1) with the major being of molecular weight 22,000 daltons containing 191 amino acid residues. A 20,000-dalton variant, which posses all known biological functions of HGH, has also been demonstrated to be important. The primary biological actions of the hormone are in direct growth promoting. HGH exerts its effect directly on target organs such as bones and muscles and indirectly through the release of somatomedins, a family of insulin-like growth factor (IGF) hormones, produced in the liver (2). In particular, somatotropin C (IGF-1) is essential for bone growth during childhood.

The clinical usefulness of the measurement of growth hormone (HGH) in children has been well established in ascertaining linear bone growth along the epiphyseal plate. Abnormal elevated levels lead to gigantism while complete absence slows the rate of growth to one third to one-half of normal. In adults, the epiphyseal growth plates have fused; HGH excess gradually produces acromegaly, a coarse thickening of the bones of the skull, hands and feet.

Immunoenzymometric assay:

In this method, calibrators, patient specimens and/or controls (containing the native hGH antigen) are first added to streptavidin coated wells. Biotinylated monoclonal and enzyme labeled antibodies are then added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of hGH.

Reaction between the various hGH antibodies and native hGH occurs in the microwells without competition or steric hindrance, forming a soluble sandwich complex.

Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody.

After equilibrium is attained, the antibody-bound fraction is separated from unbound antigen by decantation or aspiration. The activity of the enzyme present on the surface of the

well is quantitated by reaction with a suitable substrate to produce color.

The enzyme activity in the antibody-bound fraction is directly proportional to the native antigen concentration.

By utilizing several different serum references of known antigen values, a dose response curve is generated from which the antigen concentration of an unknown is ascertained.

 

The kit contains reagents for 96 tests (code LI4016).

Bring reagents to room temperature before use.

Microplate

Calibrators

HRP Conjugate

Substrate TMB

Stop Solution

Wash Buffer Conc. 50X

Adhesive Films

12x8 wells

6x1 mL/vials

1x13 mL/vial

1x16 mL/vial

1x15 mL/vial

1x20 mL/vial

2

Number of tests

96

Code

LI4016

Need assistance to make an order? Contact Sales & Orders Centre order@adaltis.net.
For Application Support application@adaltis.net