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MicroELISA

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Method & Line Sample & Target Product Package Info Product Package Info
MicroELISA Serum EIAgen Epstein Barr EBNA IgG Kit Tests per Package: 96
EIAgen The EIAgen Epstein Barr EBNA IgG Kit is an immunoenzymatic method for the qualitative determination of IgG-class antibodies to Epstein Barr nuclear antigen (EBNA) in in human serum. Code: 081017E Package: 1 Microplate
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  • Stock:
  • Not Available
    *Will be in stock within 10 days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Not Available
    *Will be in stock within 10 days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

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The EIAgen Epstein Barr EBNA IgG Kit is an immunoenzymatic method for the qualitative determination of IgG-class antibodies to Epstein Barr nuclear antigen (EBNA) in in human serum.

Epstein Barr Virus (EBV) is a herpesvirus which causes infectious mononucleosis (IM). It is also associated with Burkitt's lymphoma, nasopharyngeal carcinoma and lymphatic proliferative syndromes in immunodepressed patients. The virus is widespread throughout the world and 80-90% of the population is serum-positive.

The laboratory diagnosis of IM is traditionally performed by detecting heterophile antibodies which develop in the serum during the course of the infection, and which agglutinate horse erythrocytes. However, these antibodies may not always be present in patients affected by IM, particularly if below 14 years of age; furthermore, they may also persist for over a year after the infection. The determination of heterophile antibodies alone may therefore lead to an erroneous diagnosis. It is therefore important to determine the presence of antibodies towards the viral antigens. In particular, the detection of antibodies directed to the "Viral Capsid Antigen" (VCA) and the nuclear antigen (EBNA) is particularly useful.

During the course of IM, the IgM- and IgG-class antibodies to VCA appear early, while the IgG to EBNA develop later during the infection. The presence of IgM against VCA in the absence of IgG against EBNA therefore indicates that there is a current infection, while the presence of IgG against both VCA and EBNA is indicative of a prior infection.

The test is based on the ELISA technique (Enzyme linked Immunosorbent Assay).

The EBNA, obtained with a recombinant technique, is bound to the solid phase. Following incubation with dilute human serum, the specific immunoglobulins are bound to the antigen.

After washings to eliminate the proteins which have not reacted, incubation with the conjugate, composed of human IgG monoclonal antibodies labelled with peroxydase, is performed.

The unbound conjugate is eliminated and the peroxydase substrate is added.

The colour which develops is proportional to the concentration of specific antibodies present in the serum sample.

The kit contains reagents for 96 tests (code 081017E).

Bring reagents to room temperature before use.

Microplate

Negative Control

Positive Control

Cut-Off Control

Sorbent G

Wash Buffer Conc. 10x

Conjugate

Sample Diluent 10

Substrate TMB

Stop Solution

Adhesive Films

12x8 wells

1x1.6 mL/vial

1x1.6 mL/vial

1x2.0 mL/vial

1x7 mL/vial

2x50 mL/vials

1x16 mL/vial

2x50 mL/vials

1x12 mL/vial

1x16 mL/vial

2

Number of tests

96

Code

081017E

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