IVD SYSTEMS & AUTOMATED CLINICAL DIAGNOSTIC ANALYSERS
banner35.jpg

MicroELISA

All Reagents You Need, Available in One Click

Application Product Information & Purchase Order

Method & Line Sample & Target Product Package Info Product Package Info
MicroELISA Serum EIAgen Epstein Barr VCA IgG Kit Tests per Package: 96
EIAgen The EIAgen Epstein Barr VCA IgG Kit is an immunoenzymatic method for the qualitative/ semiquantitative determination of IgG-class antibodies to the Viral Capsid Antigen (VCA) of Epstein Barr virus in human serum. Code: 081017 Package: 1 Microplate
image not exist
  • Stock:
  • Not Available
    *Will be in stock within 10 days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Not Available
    *Will be in stock within 10 days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

Please pay attention to the revision of the document that must be the same as the revision reported in the box label.
In case of discrepancy please contact our Customer Care e-mail: info@adaltis.net.

* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.

 

The EIAgen Epstein Barr VCA IgG Kit is an immunoenzymatic method for the qualitative/ semiquantitative determination of IgG-class antibodies to the Viral Capsid Antigen (VCA) of Epstein Barr virus in human serum.

 

Epstein Barr Virus (EBV) is a herpesvirus which causes infectious mononucleosis (IM). It is also associated with Burkitt's lymphoma, nasopharyngeal carcinoma and lymphatic proliferative syndromes in immunodepressed patients. The virus is widespread throughout the world and 80-90% of the population is serum-positive.

The laboratory diagnosis of IM is traditionally performed by detecting heterophile antibodies which develop in the serum during the course of the infection, and which agglutinate horse erythrocytes. However, these antibodies may not always be present in patients affected by IM, particularly if below 14 years of age; furthermore, they may also persist for over a year after the infection. The determination of heterophile antibodies alone may therefore lead to an erroneous diagnosis. It is therefore important to determine the presence of antibodies towards the viral antigens. The detection of antibodies directed to the Viral Capsid Antigen and the nuclear antigen of the Epstein Barr virus (EBNA) is particularly useful.

During the course of IM, the IgM- and IgG-class antibodies to VCA appear early, while the IgG to EBNA develop later during the infection. The presence of IgM against VCA in the absence of IgG against EBNA therefore indicates that there is a current infection, while the presence of IgG against both VCA and EBNA is indicative of a prior infection.

The test is based on the ELISA technique (Enzyme linked Immunosorbent Assay).

The partially purified EBV antigen is bound to the solid phase. Following incubation with dilute human serum, the specific immunoglobulins are bound to the antigen.

After washings to eliminate the proteins which have not reacted, incubation with the conjugate, composed of human IgG monoclonal antibodies labelled with peroxidase, is performed.

The unbound conjugate is eliminated and the peroxidase substrate is added.

The colour which develops is proportional to the concentration of specific antibodies present in the serum sample.

The kit contains reagents for 96 tests (code 081017).

Bring reagents to room temperature before use.

Microplate

Negative Control

Calibrators

Sorbent G

Wash Buffer Conc. 10x

Conjugate

Sample Diluent 10

Substrate TMB

Stop Solution

Adhesive Films

12x8 wells

1x1.6 mL/vial

3x1.6 mL/vial

1x7 mL/vial

2x50 mL/vials

1x16 mL/vial

2x50 mL/vials

1x12 mL/vial

1x16 mL/vial

2

Number of tests

96

Code

081017

Need assistance to make an order? Contact Sales & Orders Centre order@adaltis.net.
For Application Support application@adaltis.net