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|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|MicroELISA||Serum||EIAgen Epstein Barr VCA IgM Kit||Tests per Package: 96|
|EIAgen||The EIAgen Epstein Barr VCA IgM Kit is an immunoenzymatic method for the qualitative/semiquantitative determination of IgM-class antibodies to Viral Capsid Antigen (VCA) of Epstein Barr virus in human serum.||Code: 081018||Package: 1 Microplate|
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The EIAgen Epstein Barr VCA IgM Kit is an immunoenzymatic method for the qualitative/ semiquantitative determination of IgM-class antibodies to the Viral Capsid Antigen (VCA) of Epstein Barr virus in human serum.
Epstein Barr Virus (EBV) is a herpesvirus which causes infectious mononucleosis (IM). It is also associated with Burkitt's lymphoma, nasopharyngeal carcinoma and lymphatic proliferative syndromes in immunodepressed patients. The virus is widespread throughout the world and 80-90% of the population is serum-positive.
The laboratory diagnosis of IM is traditionally performed by detecting heterophile antibodies which develop in the serum during the course of the infection, and which agglutinate horse erythrocytes. However, these antibodies may not always be present in patients affected by IM, particularly if below 14 years of age; furthermore, they may also persist for over a year after the infection. The determination of heterophile antibodies alone may therefore lead to an erroneous diagnosis. It is therefore important to determine the presence of antibodies towards the viral antigens. In particular, the detection of antibodies directed to the "Viral Capsid Antigen" (VCA) and the nuclear antigen (EBNA) is particularly useful.
During the course of IM, the IgM- and IgG-class antibodies to VCA appear early, while the IgG to EBNA develop later during the infection. The presence of IgM against VCA in the absence of IgG against EBNA therefore indicates that there is a current infection, while the presence of IgG against both VCA and EBNA is indicative of a prior infection.
The test is based on the ELISA technique (Enzyme linked Immunosorbent Assay).
The partially purified EBV antigen is bound to the solid phase. Following incubation with dilute human serum, the specific immunoglobulins are bound to the antigen. The Sorbent M serves to avoid interference by the Rheumatoid Factor.
After washings to eliminate the proteins which have not reacted, incubation with the conjugate, composed of human IgM monoclonal antibodies labelled with peroxidase, is performed.
The unbound conjugate is eliminated and the peroxidase substrate is added.
The colour which develops is proportional to the concentration of specific antibodies present in the serum sample.
The kit contains reagents for 96 tests (code 081018).
Bring reagents to room temperature before use.
Wash Buffer Conc. 10x
Sample Diluent 10
Number of tests