- Home Page
- Sales & Partners
- News & Events
- Site Map
|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|MicroELISA||Plasma,Serum||EIAgen Detect HIV 4 Total Screening Kit (480 tests)||Tests per Package: 480 tests|
|EIAgen||The EIAgen Detect HIV 4 Total Screening Kit is a 4th generation solid phase Enzyme-Linked Immunosorbent assay using a mixture of antigens and antibodies for the in vitro diagnostic screening in human serum or plasma (EDTA, Heparin and Citrate) of antibodies to HIV-1, HIV-2 and HIV-1 p24 antigen. This kit is a combined Ag/Ab assay and is not to be used for the detection of HIV-1 p24 antigen alone.||Code: 081315||Package: 5 Microplates|
Please pay attention to the revision of the document that must be the same as the revision reported in the box label.
In case of discrepancy please contact our Customer Care e-mail: email@example.com.
* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.
The EIAgen Detect HIV 4 Total Screening Kit is a 4th generation solid phase Enzyme-Linked Immunosorbent assay using a mixture of antigens and antibodies for the in vitro diagnostic screening in human serum or plasma (EDTA, Heparin and Citrate) of antibodies to HIV-1, HIV-2 and HIV-1 p24 antigen. This kit is a combined Ag/Ab assay and is not to be used for the detection of HIV-1 p24 antigen alone. This kit is for in vitro diagnostic use by a health-care professional and will not be sold to the general public.
Acquired immune deficiency syndrome (AIDS) is a set of symptoms resulting from the incapacitation of the human immune system caused by the Human Immunodeficiency Virus (HIV). HIV infection may progress to a symptomatic phase that is characterized by opportunistic infections and that may cause death.
The etiological agent of AIDS, HIV, targets specific types of T cells causing Lymphopenia and affecting T cell mediated immunity. HIV is a member of a retrovirus family with two sub-families: HIV-1 and HIV-2. HIV-1 is more virulent and transmittable than HIV-2. HIV-1 is the cause of HIV infections globally, whereas HIV-2 is found predominantly in the countries of West Africa. As serological cross-reaction between HIV-1 and HIV-2 is highly variable and dependent on the tested sample, antigens for the specific detection of both HIV-1 and HIV-2 are included in the assay.
HIV is transmitted through sexual contact with infected persons, sharing needles and syringes with infected people and transfusion of contaminated blood. Enzyme-Linked Immunosorbent Assays (such as the EIAgen Detect HIV 4 Total Screening ELISA 4th gen) are recommended for screening human blood and plasma for the presence of anti-HIV antibodies and HIV-1 p24 antigen. The presence of anti-HIV-1 and/or anti-HIV-2 in the blood indicates potential infection with HIV-1 and/or HIV-2 and consequently this blood should not be used for transfusion or for manufacture of injectable products.
Antigens representing epitopes of HIV-1 gp41 and HIV-2 gp36 are coated onto microplate wells together with monoclonal antibodies against HIV-1 p24. Serum or plasma sample is added to the well and if antibodies specific for HIV-1 and/or HIV-2 (IgG, IgM or IgA) are present in the sample, stable complexes will be formed with the HIV antigens attached to the well. HIV-1 p24 antigen, if present will bind simultaneously to the antibodies in the well and to the detector antibodies present in the Sample Diluent. Non-reactive antibodies are removed by washing. Stable antigen-antibody complexes are identified through the successive addition of biotinylated antigens and horseradish peroxidase (HRP) conjugated streptavidin. These antibody-antigen complexes are quantified through the catalytic activity of horseradish peroxidase. Peroxidase substrate solution is added and is converted to a blue-coloured product. A positive sample generates a dark blue colour while faint blue colour or colourless wells indicate a negative sample. Upon adding stopping solution, the colour of the solution will change from blue to yellow. Optical Density (OD) is measured with a spectrophotometer (ELISA reader) at 450nm with reference wavelength at 600-650nm and is in proportion to the amount of anti-HIV1/2-antibodies and HIV-1 p24 present in the sample.
The kit contains reagents for 480 tests (code 081315).
Positive Control HIV-1
Positive Control HIV-2
Positive Control HIV p-24
Conjugate # 1
Conjugate # 2 Concentrate 20x
Conjugate # 2 Diluent
Wash Buffer Concentrate 25x
Plate sealing foils
Number of tests