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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Plasma,Serum||EIAgen Detect HIV 4 Total Screening Kit (480 tests)||Tests per Package: 480 tests|
|EIAgen||The EIAgen Detect HIV 4 Total Screening Kit is a 4th generation solid phase Enzyme-Linked Immunosorbent assay using a mixture of antigens and antibodies for the in vitro diagnostic screening in human serum or plasma (EDTA, Heparin and Citrate) of antibodies to HIV-1, HIV-2 and HIV-1 p24 antigen. This kit is a combined Ag/Ab assay and is not to be used for the detection of HIV-1 p24 antigen alone.||Code: 081315||Package: 5 Microplates|
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The EIAgen Detect HIV 4 Total Screening Kit is a 4th generation solid phase Enzyme-Linked Immunosorbent assay using a mixture of antigens and antibodies for the in vitro diagnostic screening in human serum or plasma (EDTA, Heparin and Citrate) of antibodies to HIV-1, HIV-2 and HIV-1 p24 antigen. This kit is a combined Ag/Ab assay and is not to be used for the detection of HIV-1 p24 antigen alone. This kit is for in vitro diagnostic use by a health-care professional and will not be sold to the general public.
Acquired immune deficiency syndrome (AIDS) is a set of symptoms resulting from the incapacitation of the human immune system caused by the Human Immunodeficiency Virus (HIV). HIV infection may progress to a symptomatic phase that is characterized by opportunistic infections and that may cause death.
The etiological agent of AIDS, HIV, targets specific types of T cells causing Lymphopenia and affecting T cell mediated immunity. HIV is a member of a retrovirus family with two sub-families: HIV-1 and HIV-2. HIV-1 is more virulent and transmittable than HIV-2. HIV-1 is the cause of HIV infections globally, whereas HIV-2 is found predominantly in the countries of West Africa. As serological cross-reaction between HIV-1 and HIV-2 is highly variable and dependent on the tested sample, antigens for the specific detection of both HIV-1 and HIV-2 are included in the assay.
HIV is transmitted through sexual contact with infected persons, sharing needles and syringes with infected people and transfusion of contaminated blood. Enzyme-Linked Immunosorbent Assays (such as the EIAgen Detect HIV 4 Total Screening ELISA 4th gen) are recommended for screening human blood and plasma for the presence of anti-HIV antibodies and HIV-1 p24 antigen. The presence of anti-HIV-1 and/or anti-HIV-2 in the blood indicates potential infection with HIV-1 and/or HIV-2 and consequently this blood should not be used for transfusion or for manufacture of injectable products.
Antigens representing epitopes of HIV-1 gp41 and HIV-2 gp36 are coated onto microplate wells together with monoclonal antibodies against HIV-1 p24. Serum or plasma sample is added to the well and if antibodies specific for HIV-1 and/or HIV-2 (IgG, IgM or IgA) are present in the sample, stable complexes will be formed with the HIV antigens attached to the well. HIV-1 p24 antigen, if present will bind simultaneously to the antibodies in the well and to the detector antibodies present in the Sample Diluent. Non-reactive antibodies are removed by washing. Stable antigen-antibody complexes are identified through the successive addition of biotinylated antigens and horseradish peroxidase (HRP) conjugated streptavidin. These antibody-antigen complexes are quantified through the catalytic activity of horseradish peroxidase. Peroxidase substrate solution is added and is converted to a blue-coloured product. A positive sample generates a dark blue colour while faint blue colour or colourless wells indicate a negative sample. Upon adding stopping solution, the colour of the solution will change from blue to yellow. Optical Density (OD) is measured with a spectrophotometer (ELISA reader) at 450nm with reference wavelength at 600-650nm and is in proportion to the amount of anti-HIV1/2-antibodies and HIV-1 p24 present in the sample.
The kit contains reagents for 480 tests (code 081315).
Positive Control HIV-1
Positive Control HIV-2
Positive Control HIV p-24
Conjugate # 1
Conjugate # 2 Concentrate 20x
Conjugate # 2 Diluent
Wash Buffer Concentrate 25x
Plate sealing foils
Number of tests