IVD SYSTEMS & AUTOMATED CLINICAL DIAGNOSTIC ANALYSERS
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MicroELISA

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Method & Line Sample & Target Product Package Info Product Package Info
MicroELISA Plasma,Serum EIAgen HTLV Ab Kit (96 tests) Tests per Package: 96 tests
EIAgen Third generation Enzyme ImmunoAssay (ELISA) for the determination of antibodies to Human T-cell Lymphotropic Virus type I&II or HTLV I&II Ab. The kit may be used for the screening of blood units of HTLV I&II-infected patients. Code: 081210-1 Package: 1 Microplate
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  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

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* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.

Third generation Enzyme ImmunoAssay   (ELISA) for the   determination of   antibodies to Human T-cell Lymphotropic Virus type I&II or HTLV I&II Ab. The kit may be used for the screening of blood units of HTLV I&II-infected patients. For “in vitro” diagnostic use only.

HTLV I&II are retroviruses not related genetically to HIV1&2; however, they have similar routes of transmission and can have extremely long period of latency prior to manifestation of disease.

HTLV I is endemic in southern Japan, the Caribbean and the US and many other scattered population trough the world.

HTLV II is endemic in some native American populations but is detected mostly in intravenous drug users and their sexual partners.

HTLV I&II are transmitted transplacentally, parenterally, by sexual contacts and by infected blood.

ELISA has been applied to the diagnosis of HTLV I&II serology by detecting specific antibodies in plasma and sera.

Microplates are coated with HTLV I&II-specific peptides (gp46-I, gp46-II and p21-I).

The solid phase is first treated with the diluted sample and anti HTLV I&II Ab are captured, if present, by the antigens coated on the microplate.

After washing out all the other components of the sample, in the 2nd incubation bound anti HTLV I&II antibodies, IgG and IgM as well, are detected by the addition of polyclonal specific anti hIgG&M antibodies, labelled with peroxidase (HRP).

The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti HTLV I&II antibodies present in the sample.

A cut-off value let optical densities be interpreted into anti HTLV I&II antibody negative and positive results.

The kit contains reagents for 96 tests (code 081210-1).

Microplate

Negative Control

Positive Control

Calibrator

Conjugate

Assay Diluent

Sample Diluent

Substrate TMB

Stop Solution

Wash Buffer Concentrate 20x

Plate sealing foils

1

1x2 mL/vial

1x2 mL/vial

1 vial

1x16 mL/vial

1x8 mL/vial

1x50 mL/vial

1x16 mL/vial

1x15 mL/vial

1x60 mL/vial

2

Number of tests

96

Code

081210-1

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