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|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|MicroELISA||Serum||EIAgen TMPA IgG Kit||Tests per Package: 96|
|EIAgen||The EIAgen TMPA IgG Kit is an immunoenzymatic method for the qualitative determination of IgG-class antibodies to treponema pallidum in human serum.||Code: 081012D||Package: 1 Microplate|
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The EIAgen TMPA IgG Kit is an immunoenzymatic method for the qualitative determination of IgG-class antibodies to treponema pallidum in human serum.
The serological diagnosis of Syphilis is performed by demonstrating the presence of significant levels of specific Treponema pallidum antibodies in the serum sample.
The reference method used is the FTA-ABS technique which allows the detection of both IgG and IgM. However, its execution is laborious and the interpretation of the results is not simple; alternative methods have therefore been introduced to simplify the procedure. ELISA gives completely automated, reproducible and specific results, and for this reason is favoured by many operators.
The specific assay of IgM as opposed to IgG is of particular importance in the diagnosis of congenital Syphilis.
The test is based on the ELISA technique (Enzyme linked Immunosorbent Assay) (1-6).
A pool of T. pallidum antigens obtained by recombinant technique in E. Coli is bound to the solid phase (8-well strips). The specific immunoglobulins are bound to the antigen through incubation with dilute human serum.
After washings to eliminate the proteins which have not reacted, incubation is performed with the conjugate, composed of human IgG monoclonal antibodies labelled with peroxidase.
The unbound conjugate is eliminated and the peroxidase substrate is added.
The colour which develops is proportional to the concentration of specific antibodies present in the serum sample.
The kit contains reagents for 96 tests (code 081012D).
Bring reagents to room temperature before use.
Wash Buffer Conc. 10x
Sample Diluent 10
Number of tests