IVD SYSTEMS & AUTOMATED CLINICAL DIAGNOSTIC ANALYSERS
banner35.jpg

MicroELISA

All Reagents You Need, Available in One Click

Application Product Information & Purchase Order

Method & Line Sample & Target Product Package Info Product Package Info
MicroELISA Serum EIAgen PSA Equi Kit Tests per Package: 96
EIAgen EIAgen PSA Equi Kit is a direct solid phase enzyme immunoassay for the quantitative measurement of Total Prostate Specific Antigen (tPSA) in human serum. Code: LI4027CF1 Package: 1 Microplate
image not exist
  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

Please pay attention to the revision of the document that must be the same as the revision reported in the box label.
In case of discrepancy please contact our Customer Care e-mail: info@adaltis.net.

* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.

EIAgen PSA Equi Kit is a direct solid phase enzyme immunoassay for the quantitative measurement of Total Prostate Specific Antigen (tPSA) in human serum.

Prostate Specific antigen (PSA) is a serine protease with chymotrypsin-like activity (1,2). The protein is a single chain glycoprotein with a molecular weight of 28.4 kDA (3). PSA derives its name from the observation that it is a normal antigen of the prostrate but is not found in any other normal or malignant tissue.

PSA is found in benign, malignant and metastatic prostrate cancer. Since prostate cancer is the second most prevalent form of male malignancy, the detection of elevated PSA levels plays an important role in the early diagnosis. Serum PSA levels have been found too more useful than prostatic acid phosphatase (PAP) in the diagnosis and management of patients due to increased sensitivity (4).

In this method, PSA calibrator, patient specimen or control is first added to a streptavidin coated well. Biotinylated monoclonal and enzyme labeled antibodies (directed against distinct and different epitopes of PSA) are added and the reactants mixed. Reaction between the various PSA antibodies and native PSA forms a sandwich complex that binds with the streptavidin coated to the well.

After the completion of the required incubation period, the enzyme-PSA antibody bound conjugate is separated from the unbound enzyme-PSA conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color.

The employment of several serum references of known prostate specific antigen (PSA) levels permits the construction of a dose response curve of activity and con­centration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with PSA concentration.

Immunoenzymometric assay:

The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (enzyme and immobilized), with different and distinct epitope recognition, in excess, and native antigen. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-PSA antibody.

Upon mixing monoclonal biotinylated antibody, the enzyme-labeled antibody and a serum containing the native antigen, reaction results between the native antigen and the antibodies, without competition or steric hindrance, to form a soluble sandwich complex.

Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody.

After equilibrium is attained, the antibody‑bound fraction is separated from unbound antigen by decantation or aspiration. The enzyme activity in the antibody‑bound fraction is directly proportional to the native antigen concentration. By utilizing several different serum references of known antigen values, a dose response curve can be generated from which the anti­gen concentration of an unknown can be ascertained.

The kit contains reagents for 96 tests (code LI4027CF1).

Bring reagents to room temperature before use.

Microplate

Calibrators

Conjugate

Substrate TMB

Stop Solution

Wash Buffer Conc. 50X

Adhesive Films

12x8 wells

6x1 mL/vials

1x13 mL/vial

1x16 mL/vial

1x15 mL/vial

1x20 mL/vial

2

Number of tests

96

Code

LI4027CF1

 

Need assistance to make an order? Contact Sales & Orders Centre order@adaltis.net.
For Application Support application@adaltis.net