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|Method & Line||Sample & Target||Product||Package Info||Product||Package Info|
|MicroELISA||Serum||EIAgen Measles IgM Kit||Tests per Package: 96|
|EIAgen||The EIAgen Measles IgM Kit is an immunoenzymatic capture method for the qualitative determination of IgM-class antibodies to measles virus in human serum.||Code: 081023DS||Package: 1 Microplate|
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The EIAgen Measles IgM Kit is an immunoenzymatic capture method for the qualitative determination of IgM-class antibodies to measles virus in human serum.
Measles has been largely eliminated in recent years as a major childhood disease thanks to immunisation campaigns. However, as a result of vaccination failure or subjects who have not been vaccinated, there has been a shifting tendency for young adults to become infected. Measles, a paramyxovirus, causes a very contagious respiratory infection which can have severe consequences, particularly in adults. The screening of pregnant women, young adults and other high risk patients for circulating antibodies is important in determining the immune status.
The test for the assay of Anti anti-Measles IgM is based on the principle of the capture of these immunoglobulins by anti-human IgM monoclonal antibodies bound on the solid phase. A subsequent incubation with measles antigens in a complex with monoclonal antibodies conjugated to Horse Radish Peroxydase (HRP) selects the IgM antibodies specific for the antigen and is revealed by the addition of the peroxydase substrate. When the enzymatic reaction is stopped by the addition of a sulphuric acid solution, a yellow colouring forms. The colour, which is proportional to the amount of specific antibodies present in the sample, can be read in an ELISA microplate reader.
The kit contains reagents for 96 tests (code 081023DS).
Bring reagents to room temperature before use.
Wash Buffer Conc. 10x
Sample Diluent 2
Measles Virus Antigen
Number of tests