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|Method & Line||Sample & Target||Product||Package Info|
|Clinical Chemistry||Plasma,Serum,Urine||MAGNESIUM||Tests per Package: 912|
|Pchem Reagents||The Pchem Magnesium Reagent Kit has been designed for use in the quantitative determination in vitro of the concentration of Magnesium in human serum, plasma and urine. The results of the test must always be interpreted in conjunction with the clinical picture.||Code: ADA-R0200000801||Package: R1 4 x 48.5 mL|
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The Pchem Magnesium Reagent Kit has been designed for use in the quantitative determination in vitro of the concentration of Magnesium in human serum, plasma and urine. The results of the test must always be interpreted in conjunction with the clinical picture.
This kit is intended for In vitro diagnostic use only, must not be sold to the general public and the test has to be carried out by a health-care professional person.
The kit contains reagents sufficient for 912 tests.
About 55% of the total magnesium present in the human body is found in the bone, while the remaining portion is within the cells, bound to proteins or molecules with a negative charge. About 55% of the magnesium present in the serum is free, 30% is bound to proteins and 15% is in the form of compounds bound to phosphate or citrate ions. It is of fundamental importance in oxidative phosphorylation, in glycolysis and in cellular replication. The reference method for the determination of magnesium in serum and urine makes use of atomic absorption spectrophotometry. For routine diagnostic use, colorimetric assay methods have been developed based on compounding reactions between colouring substances (calmagite, methylthymol blue, titanium yellow, non sulphonated derivatives of xylidyl-blue, xylidyl-blue). The I.S.E. S.r.l. reagent makes use of xylidyl blue which is more sensitive than other stains. Like the other colouring substances, it also compounds cations other than magnesium, and in particular calcium, and it must therefore be associated with a chelating agent which eliminates this type of interference (1.2).
Xylidyl-blue forms a soluble coloured compound with magnesium in an alkaline ambient, with a maximum absorbance between 505 and 520 nm. The intensity of the colour of the Mg-xylidyl-blue compound is directly correlated to the magnesium concentration in the sample.
Pchem Magnesium Reagent Kit
Reagent (R) – 4 x 48.5 mL/vial