IVD Reagents

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The Pchem Phosphorus Reagent Kit has been designed for use in the quantitative determination in vitro of the Inorganic Phosphorus concentration in human serum or urine. The results of the test must always be interpreted in conjunction with the clinical picture.

This kit is intended for In vitro diagnostic use only, must not be sold to the general public and the test has to be carried out by a health-care professional person.

The kit contains reagents sufficient for 1369 tests.

Phosphorus is an important element which is present in the human organism in the form of inorganic or organic phosphate. It is present in the serum in the form of both monovalent and divalent phosphate anions. About 10% of serum phosphate is bound to proteins, 35% is found in combination with sodium, calcium and magnesiun, the remaining amount is free. Phosphate represents one of the main components of hydroxyapatite which is present in bone, it has an important role as a high-energy phosphate bond and is a vital component for numerous enzymatic systems. Low blood phosphate concentrations are relatively common in the hospitalized population, due to several causes such as: stimulation of insulin secretion which favours the transport of phosphates in the cells and respiratory alkalosis. A loss of phosphates through the kidneys can cause low serum phosphate levels. High blood phosphate levels are normally correlated to renal insufficiency. A decrease in glomerular filtration limits phosphate excretion through the kidneys with the consequent development of high blood phosphate levels.

The method measures the absorbance at 340 nm of non-reduced phospho-molibdate and allows the determination of inorganic phosphates present in the sample. In the presence of sulphuric acid, inorganic phosphorus reacts with ammonium molibdate to give an unreduced complex which can be read at 340 nm.

Inorganic Phosphorus + H₂SO₄ + ammonium molibdate = Phospho-molibdate complex.

Pchem Phosphorus Reagent Kit

Code ADA-R0100000701

Reagent (R) – 6 x 48.5 mL/vial