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|Method & Line||Sample & Target||Product||Package Info|
|Clinical Chemistry||Plasma,Serum||HDL CHOLESTEROL||Tests per Package: 367|
|Pchem Reagents||The HDL Cholesterol Reagent Kit has been designed for use in the quantitative determination in vitro of the concentration of HDL cholesterol in in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.||Code: ADA-R0200001301||Package: R1 2 x 30 mL; R2 2 x 10 mL|
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The HDL Cholesterol Reagent Kit has been designed for use in the quantitative determination in vitro of the concentration of HDL cholesterol in in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.
This kit is intended for In vitro diagnostic use only, must not be sold to the general public and the test has to be carried out by a health-care professional person.
The kit contains reagents sufficient for 367 tests.
Cholesterol is in part synthesized in the organism and is in part introduced through the diet. It is an essential component of the cell membrane and lipoproteins and is a precursor for the synthesis of steroid hormones and biliary acids. About 25% of the total cholesterol present in the serum is transported by lipoproteins in the high density fraction (HDL, High Density Lipoproteins). About 50% of the HDL mass is composed of proteins, 30% of phospholipids and the remaining 20% of cholesterol. The liver and intestine are both involved in the production of HDL which seems to play an important role in the efflux of cholesterol from peripheral tissues, reducing the amount of cholesterol stored therein. HDL also have a role in the mechanism of bringing back the cholesterol from the peripheral tissue to the liver to be eliminated and transformed into biliary acids, this metabolic mechanism being know by the term “inverse transport of cholesterol”. As there is an inverse correlation between the HDL plasma concentration and the incidence of coronary heart disease, an accurate determination of the HDL cholesterol is essential to establish eventual risk factors related to coronary heart disease. The various techniques available for determination of HDL cholesterol levels include ultracentrifugation, electrophoresis, HPLC (high performance liquid chromatography) and methods based on precipitation. For all these reasons, from the clinical aspect the need was felt to develop a pratical, reliable method which does not require preliminary treatments.
The HDL-L reagent is produced using a combination of detergents and phosphorus compounds which specifically bind LDL, VLDL and CM (chilomicrons) but not HDL. This combination impedes LDL, VLDL and CM from reacting with CO (cholesterol oxidase) and CE (cholesterol esterase), while HDL-cholesterol is able to react with both enzymes.
Pchem HDL Cholesterol Reagent Kit
Reagent 1 (R1) – 2 x 30 mL/vial
Reagent 2 (R2) – 2 x 10 mL/vial