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|Method & Line||Sample & Target||Product||Package Info|
|Clinical Chemistry||Plasma,Serum||LDL CHOLESTEROL||Tests per Package: 182|
|Pchem Reagents||The LDL Cholesterol Reagent Kit has been designed for use in the quantitative determination in vitro of the concentration of LDL cholesterol in in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.||Code: ADA-R0200001401||Package: R1 1 x 30 mL; R2 1 x 10 mL|
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The LDL Cholesterol Reagent Kit has been designed for use in the quantitative determination in vitro of the concentration of LDL cholesterol in in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.
This kit is intended for In vitro diagnostic use only, must not be sold to the general public and the test has to be carried out by a health-care professional person.
The kit contains reagents sufficient for 182 tests.
Total cholesterol in humans is principally distributed between three major classes of lipoproteins: VLDL (very low density lipoproteins), LDL (low density lipoproteins) and HDL (high density lipoproteins). An increase in the plasma levels of LDL cholesterol constitutes one of the major risk factors for the development of coronary heart disease (CHD). In humans, LDL are carriers which transport two thirds of the blood cholesterol, having an important role in the formation of aterosclerotic plaques. LDL catabolism takes place in the liver and peripheral tissues following interaction with specific highaffinity LDL receptors. The presence of these receptors has been demonstrated in most cells, although they are more numerous in certain types, e.g. adrenocortical cells in which LDL cholesterol acts as principle substrate for the synthesis of steroid hormones. A defect in the process of removal of LDL or the superposition of low density lipoproteins, precursors of LDL, can cause an increased concentration of LDL in the serum. The precipitation method for the determination of LDL cholesterol is little used because the results are often inaccurate in the presence of high serum triglycerides levels. The concentration of LDL cholesterol is measured in the serum or plasma collected from the patient after fasting, taking three different parameters: total cholesterol, HDL cholesterol and total triglycerides. The accuracy of this formula is due to the fact that the serum levels of triglycerides are closely correlated to the VLDL concentrations. The I.S.E. S.r.l. method is based on the selective solubilization of LDL cholesterol by an ionic detergent and the interaction of some lipoprotein components (VLDL and chilomicrons).
The LDL-L reagent is produced using a combination of detergents and phosphorus compounds which specifically bind HDL, VLDL and CM (chilomicrons) but not LDL. This combination impedes HDL, VLDL and CM from reacting with CO (cholesterol oxidase) and CE (cholesterol esterase), while LDL-cholesterol is able to react with both enzymes.
The compound (Quinone dye) which forms is read at lambda 578 nm, develops a colour, the intensity of which is proportional to the LDL concentration in the test sample.
Pchem LDL Cholesterol Reagent Kit
Reagent 1 (R1) – 1 x 30 mL/vial
Reagent 2 (R2) – 1 x 10 mL/vial