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|Method & Line||Sample & Target||Product||Package Info|
|Clinical Chemistry||Plasma,Serum||UREA||Tests per Package: 1125|
|Pchem Reagents||The Pchem Urea Reagent Kit has been designed for use in the quantitative determination in vitro of the Urea concentration in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.||Code: ADA-R0200000901||Package: R1 4 x 48.5 mL; R2 4 x 13.0 mL|
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The Pchem Urea Reagent Kit has been designed for use in the quantitative determination in vitro of the Urea concentration in human serum or plasma. The results of the test must always be interpreted in conjunction with the clinical picture.
This kit is intended for In vitro diagnostic use only, must not be sold to the general public and the test has to be carried out by a health-care professional person.
The kit contains reagents sufficient for 1125 tests.
Urea is the product of metabolism with the highest nitrogen content deriving from protein catabolism in the human species, and represents over 75% of protein nitrogen. Over 90% of Urea is secreted by the kidneys, while the gastrointestinal tract and the skin account for most of the remaining fraction. There are three factors which determine the plasma urea concentration: renal perfusion and amount of water secreted, rate of urea synthesis, and rate of glomerular filtration. Numerous increases in the plasmatic urea concentration are caused by a wide range of renal diseases. In children, protein catabolism is reduced due to the greater protein requirements necessary for growth, therefore the serum urea concentrations are lower compared to adults. Pregnant women show urea levels outside the normal range due to the greater protein requirements of the fetus, in addition to the renal hyperperfusion.
In the presence of urease, urea is hydrolyzed into ammonium ions and carbon dioxide. In the presence of glutamate-dehydrogenase (GLDH), the ammonium ion produced reacts with alpha-ketoglutarate (alpha-KG) and NADH to form glutamate and NAD. The consumption of NADH over a given period of time, determined at lambda 340 nm, is proportional to the Urea concentration in the test sample.
Pchem Urea Reagent Kit
Reagent 1 (R1) – 4 x 48.5 mL/vial
Reagent 2 (R2) – 4 x 13.0 mL/vial