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|Method & Line||Sample & Target||Product||Package Info|
|IMMUNOgen - Immunoassays||Plasma,Serum||Androstenedione||Tests per Package: 100|
|IMMUNOgen||The Eclectica Androstenedione assay for the Eclectica and Eclectica TiCA analysers has been designed for the quantitative determination of Androstenedione in human serum and heparinized plasma. The method can be used to measure Androstenedione concentrations over the range of 0.05 to 15 µg/ml (0.175–52.37 nmol/l) of Androstenedione.||Code: 1700100||Package: 2 x 50|
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The Eclectica Androstenedione assay for the Eclectica analyzers has been designed for the quantitative determination of Androstenedione in human serum and heparinized plasma. The method can be used to measure Androstenedione concentrations over the range of 0.05 to 15 µg/ml (0.175–52.37 nmol/l) of Androstenedione.
Androstenedione (ASD, 4-Androstene-3,17-dione), a C19 steroid, is produced in the adrenal gland and gonads. ASD is an immediate precursor to both testosterone and estrone, both of which may be subsequently converted to estradiol. Due to the presence of a 17-oxo (rather than hydroxyl) group, ASD has relatively weak androgenic activity, estimated at <20% of testosterone . Although it is a weak androgen, serum ASD levels may exceed testosterone in both normal and disease states, ASD secretion and production rates exceed those of testosterone in women, and significant extra-adrenal conversion of ASD to testosterone occurs. Furthermore, the affinity of sex hormone-binding globulin for ASD is much less than for testosterone or estradiol [1-3].
The physiologic role of ASD is not well defined. Serum ASD levels are high in fetal and neonatal serum, decrease during childhood, and increase during puberty. In normal pubertal and adult men, the major portion of ASD is derived from the testis, either directly or from conversion of testosterone, while in normal adult women essentially equivalent amounts of ASD are produced by the adrenal gland and ovary [2,3]. Increased ASD levels may play a role in the development of secondary sexual hair during adrenarche. Serum ASD levels show significant diurnal variation dependent on the secretion of ACTH. Ovarian ASD production is stimulated by luteinizing hormone, and serum ASD levels vary with the menstrual cycle . Adrenal ASD production gradually declines with advanced age in both men and women. In addition, ovarian ASD production decreases after menopause .
Measurement of serum ASD provides a useful marker of androgen biosynthesis. Elevated ASD levels have been demonstrated in virilizing congenital adrenal hyperplasia; additionally ASD levels may have advantages over 17-hydroxy-progesterone levels in monitoring treatment of this condition, e.g. less marked diurnal variation and less suppression after brief glucocorticoid exposure . Serum ASD levels are also increased in polycystic ovary syndrome, ovarian stromal hyperthecosis, 3ß-hydroxysteroid dehydrogenase deficiency, and other causes of hirsutism in women [3,6,7]. By definition, ASD levels are normal in idiopathic hirsutism. Elevated serum ASD levels may also occur in adrenal and ovarian virilizing tumors . In a prospective study of over 1000 men, a dose-response relationship of androstenedione and prostate cancer risk was demonstrated ; additional studies will be needed to confirm these findings.
Assays for ASD include gas-liquid chromatography, mass spectrometry, and immunoassay. The Adaltis ASD Assay Kit uses a specific and sensitive rabbit anti-human ASD polyclonal antiserum [6-8]. Cross-reactivity to DHEA, DHEA-S, testosterone and androgen metabolites is negligible.
The Eclectica assays for the Eclectica analyzers consists of:
In the Eclectica Androstenedione assay, a rabbit anti-Androstenedione antibody is used in an enzyme immunoassay system which incorporates magnetic solid phase separation. All incubation times and reagent volumes are determined by an assay specific software protocol.
Fixed amounts of fluorescein-conjugated anti-Androstenedione antibody and a Androstenedione derivative conjugated to alkaline phosphatase are added to a sample, control, or standard. The reactants are incubated at 37°C. During the incubation Androstenedione in the sample,control or calibrators competes with the derivative for binding to the anti-Androstenedione antibody.
At the end of the incubation period anti-fluorescein coupled to a magnetic solid phase is added in excess. This rapidly and specifically binds to the Androstenedione antibody complex and is sedimented in a magnetic field.
After aspirating the liquid phase and washing the solid phase, a solution of the enzyme substrate, phenolphthalein monophosphate is added to the incubation cell and the each cell incubated at 37°C. After incubation the enzyme reaction is stopped by the addition of a stop reagent and the intensity of the color developed is measured photometrically. The intensity of the color developed is inversely proportional, within the working range of the assay, to the concentration of Androstenedione in the sample. The concentration of Androstenedione in a patient sample or control is then determined by interpolation from a stored standard curve.
Eclectica Androstenedione Reagent Kit
Eclectica Androstenedione Calibration Kit
Eclectica Immunoassay Common Reagents Kit
Eclectica Immunoassay Wash Solution
Eclectica Systemic Wash Solution
1700006 Eclectica Cleaning Kit