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Method & Line Sample & Target Product Package Info
IMMUNOgen - Immunoassays Plasma,Serum Estradiol Tests per Package: 100
IMMUNOgen The Eclectica Estradiol assay for the Eclectica and Eclectica TiCA analysers has been designed for the quantitative determination of Estradiol in human serum and heparinized plasma. The method can be used to measure Estradiol concentrations over the range of 10 to 1600 pg/ml (36,7 – 5824 pmol/l). Code: 1702600 Package: 2 x 50
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  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

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The Eclectica Estradiol assay for the Eclectica analyzers has been designed for the quantitative determination of Estradiol in human serum and heparinized plasma. The method can be used to measure Estradiol concentrations over the range of 10 to 1600 pg/ml (36,7 – 5824 pmol/l).

Estradiol (estra-1, 3, 5 (10)-triene-3, 17ß-diol) is a C18 steroid hormone with a molecular weight of 272.4 daltons. It is the most potent natural estrogen, produced mainly by the Graffian follicle of the female ovary and the placenta, and in smaller amounts by the adrenals, and the male testes (1,2,3).

Estradiol (E2) is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG), and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates as free hormone or in the conjugated form (4,5). Estrogenic activity is effected via estradiol-receptor complexes which trigger the appropriate response at the nuclear level in target sites. These sites include the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin.

In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation (6,7). The rising estradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinising hormone (LH), which are essential for follicular maturation and ovulation, respectively (8,9). Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy maternal serum estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained through pregnancy (10).

Serum estradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls (11), and primary and secondary amenorrhea and menopause (12). Estradiol levels have been reported to be elevated in the presence of estradiol secreting tumors (13). In male subjects, serum estradiol levels have been reported to be increased in patients with feminising syndromes (14), gynaecomastia (15), and testicular tumours (16).

In cases of infertility, serum estradiol measurements are useful for monitoring induction of ovulation following treatment with, for example, clomiphene citrate, LH-releasing hormone (LH-RH), or exogenous gonadotropins (17,18). During ovarian hyperstimulation for in vitro fertilisation (IVF), serum estradiol concentrations are usually monitored daily for optimal timing of human chorionic gonadotropin (hCG) administration and oocyte collection (19).

The Eclectica assays for the Eclectica analyzers consists of:

  1. Eclectica Estradiol Reagent Kit: specific reagents of Eclectica Estradiol kit to execute the test and to verify the standardization.
  2. Eclectica Estradiol Calibration Kit: for standardization.
  3. Eclectica Diluent 3 Kit: for samples dilution at high concentration.
  4. Eclectica Immunoassay Common Reagents Kit: for general chemistry reaction.
  5. Eclectica Immunoassay Wash Solution: Wash Solution specific for Immunological reaction.
  6. Eclectica Systemic Wash Solution: Wash Solution of Eclectica system.

In the Eclectica Estradiol assay, a rabbit polyclonal anti-estradiol antibody is used in an enzyme immunoassay system which incorporates magnetic solid phase separation. All incubation times and reagent volumes are determined by an assay specific software protocol.

Fixed amounts of fluorescein-conjugated anti-Estradiol antibody and a Estradiol derivative conjugated to alkaline phosphatase are added to a sample, control, or standard. The reactants are incubated at 37°C. During the incubation Estradiol in the sample, control or calibrators competes with the derivative for binding to the anti-Estradiol antibody.

At the end of the incubation period anti-fluorescein coupled to a magnetic solid phase is added in excess. This rapidly and specifically binds to the estradiol antibody complex and is sedimented in a magnetic field.

After aspirating the liquid phase and washing the solid phase, a solution of the enzyme substrate, phenolphthalein monophosphate is added to the incubation cell and the each cell incubated at 37°C. After incubation the enzyme reaction is stopped by the addition of a stop reagent and the intensity of the color developed is measured photometrically. The intensity of the color developed is inversely proportional, within the working range of the assay, to the concentration of Estradiol in the sample. The concentration of Estradiol in a patient sample or control is then determined by interpolation from a stored standard curve.

Eclectica Estradiol Reagent Kit

Eclectica Estradiol Calibration Kit

Eclectica Diluent 3 Kit

Eclectica Immunoassay Common Reagents Kit

Eclectica Immunoassay Wash Solution

Eclectica Systemic Wash Solution

1700006 Eclectica Cleaning Kit

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