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Method & Line Sample & Target Product Package Info
IMMUNOgen - Immunoassays Plasma,Serum,Urine FSH Tests per Package: 100
IMMUNOgen The Eclectica FSH assay for the Eclectica and Eclectica TiCA analysers has been designed for the quantitative determination of follicle stimulating hormone (Follitropin,FSH) in human serum, heparinized plasma and urine. The method can be used without dilution for samples containing 0.5 to 150 mIU/ml of FSH. Primary calibration is against the WHO I.R.P. (94/632) and against WHO 1st I.S. (92/510) for immunoassay of pituitary FSH. Code: 1701000 Package: 2 x 50
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  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
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  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

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The Eclectica FSH assay for the Eclectica analyzers has been designed for the quantitative determination of follicle stimulating hormone (Follitropin,FSH) in human serum, heparinized plasma and urine. The method can be used without dilution for samples containing 0.5 to 150 mIU/ml of FSH. Primary calibration is against the WHO I.R.P. (94/632) and against WHO 1st I.S. (92/510) for immunoassay of pituitary FSH (1).

Follicle stimulating hormone (FSH) is a glycoprotein hormone produced by the pituitary gland with a molecular weight of approximately 32,000. It consists of two non-covalently associated dissimilar subunits, alpha and beta (1). The alpha subunit of human FSH, is of 89 amino acids and is closely similar in its structure of the alpha chains of luteinizing hormone (LH) and thyroid stimulating hormone (TSH). The alpha chain of chorionic gonadotrophin is also closely related, although it contains 92 amino acids (2, 3). The beta subunit has 115 amino acids and confers the distinctive biological and immunological specificity on the whole molecule. Neither subunit is active on its own. The beta subunit controls the binding of the intact hormone to its specific receptors on target cells, and the alpha subunit then activates the cellular messenger, probably adenyl cyclase (4).

The main action of FSH is to promote follicular development in the ovary and gametogenesis in the testis. It is secreted by the basophillic cells of the anterior pituitary, under the control of other hormones. Secretion is stimulated by gonadotrophin releasing hormone (GnRH) from the hypothalamus (5, 6) and is inhibited by the sex steroids estradiol, progesterone and testosterone and also by inhibin (7, 8). The release of FSH by the pituitary is biphasic: there is a steady basal production with episodic pulses (9, 10).

During the menstrual cycle, circulating FSH levels are generally inversely related to those of estradiol, progesterone, and inhibin. This pattern of generally negative feedback is reversed shortly before ovulation when a significant increase in both FSH and LH is seen (11). After ovulation and conversion of the follicle to a corpus luteum, negative feedback is again the main influence in FSH secretion (4, 12). At the menopause, when ovarian function declines, circulating FSH levels rise significantly as a result of the removal of the negative feedback affect of estradiol on both pituitary and hypothalamus (13).

In the male, FSH together with LH and Testosterone is responsible for the development and maintenance of spermatogenesis in the seminiferous tubules of the testis (14). Infertility in males may be due to hypogonadism as a result of primary testicular failure. Lack of negative feedback by testosterone in these cases gives rise to elevated levels of FSH, (15) also characteristics of Klinefelter’s syndrome. FSH levels tend to rise with age, especially in men over 50, as a result of progressive decline in gametogenesis (16).

Low levels of FSH may indicate dysfunction of the hypothalamic/pituitary axis. Hypopituitarism can result in secondary gonadal failure and can therefore be a cause of infertility in both men and women. Determination of circulating FSH after administration of GnRH may be useful in evaluating pituitary function and in the differential diagnosis of hypothalamic and pituitary disorders in cases of amenorrhea or hypogonadism associated with low or normal FSH concentration (17).

The measurement of FSH in urine provides a simple, non-invasive sampling method that may be useful as a reflection of pituitary function (18).

The Eclectica assays for the Eclectica analyzers consist of:

  1. Eclectica FSH Reagent Kit: specific reagents of Eclectica FSH kit to execute the test and to verify the standardization.
  2. Eclectica FSH Calibration Kit: for standardization.
  3. Eclectica Immunoassay Common Reagents Kit: for general chemistry reaction.
  4. Eclectica Immunoassay Wash Solution: Wash Solution specific for Immunological reaction.
  5. Eclectica Systemic Wash Solution: Wash Solution of Eclectica system.

In the Eclectica FSH assay, two high affinity monoclonal antibodies are used in an immunoenzymetric assay system which incorporates magnetic solid phase separation. All incubation times and reagent volumes are determined by an assay specific software protocol.

Fixed amounts of fluorescein-conjugated anti-FSH monoclonal antibody and anti-FSH monoclonal antibody conjugated to alkaline phosphatase are added to a sample, control, or standard. The reactants are incubated at 37°C. During the incubation fluorescein-anti-FSH monoclonal antibody binds to a discrete site on the FSH molecule. Alkaline phosphatase anti-FSH monoclonal antibody binds to a second site on the FSH molecule forming a sandwich.

At the end of the incubation period anti-fluorescein coupled to a magnetic solid phase is added in excess. This rapidly and specifically binds to the FSH monoclonal antibody complex and is sedimented in a magnetic field.

After aspirating the liquid phase and washing the solid phase, a solution of the enzyme substrate, phenolphthalein monophosphate is added to the incubation cell and the each cell incubated at 37°C. After incubation the enzyme reaction is stopped by the addition of a stop reagent and the intensity of the color developed is measured photometrically.The intensity of the color developed is directly proportional, within the working range of the assay, to the concentration of FSH in the sample. The concentration of FSH in a patient sample or control is then determined by interpolation from a stored standard curve.

Eclectica FSH Reagent Kit

Eclectica FSH Calibration Kit

Eclectica Immunoassay Common Reagents Kit

Eclectica Immunoassay Wash Solution

Eclectica Systemic Wash Solution

1700006 Eclectica Cleaning Kit

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