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|Method & Line||Sample & Target||Product||Package Info|
|IMMUNOgen - Immunoassays||Plasma,Serum||Testosterone||Tests per Package: 100|
|IMMUNOgen||The Eclectica Testosterone assay for the Eclectica and Eclectica TiCA analysers has been designed for the quantitative determination of Testosterone in human serum and heparinized plasma. The method can be used to measure Testosterone concentrations over the range of 0.1 to 17 ng/ml (0.35 - 59 nmol/l).||Code: 1706000||Package: 2 x 50|
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The Eclectica Testosterone assay for the Eclectica analyzers has been designed for the quantitative determination of Testosterone in human serum and heparinized plasma. The method can be used to measure Testosterone concentrations over the range of 0.1 to 17 ng/ml (0.35 - 59 nmol/l).
Testosterone (17ß-hydroxy-4-androsten-3-one) is a C19 steroid hormone with a molecular weight of 288.4 daltons.
It is the principal male hormone produced by the interstitial (Leydig) cells of the male testes and in smaller amounts by the adrenals and the female ovaries (1, 2, 3).
Testosterone is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG), and with lower affinity to other serum proteins such as cortisol binding globulins (CBG) and albumin (4).
Testicular function is controlled by two pituitary hormones, follicle stimulating hormone (FSH) and luteinising hormone (LH). LH however exerts the primary control on testosterone production by acting directly on the Leydig cells causing them to secrete testosterone. Testosterone in males is responsible for the development and primary male differentiation of the fetus, and later at puberty, for the growth, development and maintenance throughout life of the male reproductive system and secondary sexual characteristics (2).
In males serum testosterone measurements are made to investigate and evaluate hypogonadism, adrenal/gonadal tumors, early or delayed puberty, testicular feminisation, congenital disorders such as Klinefelters syndrome, adrenal hyperplasia or ambiguous genitalia in the neonate (2, 5, 6).
In females testosterone measurements are used primarily to diagnose virilization associated possibly with androgen administration or over production, polycystic ovarian disease and hirsutism (7, 8, 9).
The Eclectica assays for the Eclectica analyzers consists of:
In the Eclectica Testosterone assay, a rabbit polyclonal anti-testosterone antibody is used in an enzyme immunoassay system which incorporates magnetic solid phase separation. All incubation times and reagent volumes are determined by an assay specific software protocol.
Fixed amounts of fluorescein-conjugated anti-Testosterone antibody and a Testosterone derivative conjugated to alkaline phosphatase are added to a sample, control, or standard. The reactants are incubated at 37°C. During the incubation Testosterone in the sample, control or calibrators competes with the derivative for binding to the anti-Testosterone antibody.
At the end of the incubation period anti-fluorescein coupled to a magnetic solid phase is added in excess. This rapidly and specifically binds to the testosterone antibody complex and is sedimented in a magnetic field.
After aspirating the liquid phase and washing the solid phase, a solution of the enzyme substrate, phenolphthalein monophosphate is added to the incubation cell and the each cell incubated at 37°C. After incubation the enzyme reaction is stopped by the addition of a stop reagent and the intensity of the color developed is measured photometrically. The intensity of the color developed is inversely proportional, within the working range of the assay, to the concentration of Testosterone in the sample. The concentration of Testosterone in a patient sample or control is then determined by interpolation from a stored standard curve.
Eclectica Testosterone Reagent Kit
Eclectica Testosterone Calibration Kit
Eclectica Immunoassay Common Reagents Kit
Eclectica Immunoassay Wash Solution
Eclectica Systemic Wash Solution
1700006 Eclectica Cleaning Kit