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Method & Line Sample & Target Product Package Info
IMMUNOgen - Immunoassays Serum Total T3 Tests per Package: 100
IMMUNOgen The Eclectica T3 assay for the Eclectica and Eclectica TiCA analysers has been designed for the quantitative determination of Triiodothyronine (T3) in human serum. The method can be used to measure concentrations over the range of 0.3 to 10 ng/ml. Code: 1708400 Package: 2 x 50
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  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

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The Eclectica T3 assay for the Eclectica analyzers has been designed for the quantitative determination of Triiodothyronine(T3) in human serum. The method can be used to measure concentrations over the range of 0.3 to 10 ng/ml.

Thyroxine (T4) and 3, 5, 3'-triiodothyronine (T3) are the two principal thyroid hormones. In contrast to T4, which is exclusively a product of the thyroid gland, T3 is mainly produced in the peripheral tissues by 5'-monodeiodination of circulating T4. In normal adults about 80% of T3 is produced by peripheral conversion and about 20% is secreted directly by the thyroid (1). A small amount of T4 is 5-monodeiodinated to form 3,3',5'-triiodothyronine (reverse T3): this is probably biologically inactive (2). The proportion of T4 converted to reverse T3 may be increased under certain circumstances, such as severe illness or medication with Amiodarone or radiographic contrast agents (3,4,5). This reduces the production and hence the circulating level of T3. Considerable evidence exists to show that T3 is the more active thyroid hormone and some authors have suggested that T4 has no intrinsic activity (6).

Both T3 and T4 circulate in association with serum proteins, the most important of which is thyroxine binding globulin (TBG). This has a higher affinity for T4 than for T3. Approximately 80% of circulating T3 is bound to TBG. Most of the rest is bound to prealbumin and albumin; only about 0.5% of T3 is "free" in the serum (i.e. not bound to serum proteins) (7).

The concentration of T3 in serum can be altered by a change in the activity of the thyroid gland or by alteration in the peripheral metabolism of T4. The thyroid is under the control of thyroid stimulating hormone (TSH) which in turn is regulated by hypothalamic thyrotrophin-releasing hormone (TRH). T3 exerts a negative feedback control on the release of TSH which is principally at the level of the pituitary. A slight elevation in T3 levels above normal will block TSH release in normal subjects; this block cannot be overcome by increasing doses of TRH (8).

Measurement of the concentration of T3 in serum is most useful in the diagnosis of hyperthyroidism. In the majority of patients with this condition, circulating total T4 and total T3 are both elevated. In some cases, however, the hyperthyroidism results solely from an increased production of T3, a condition referred to as "T3 thyrotoxicosis"(9).

Elevated T3 levels with normal T4 may also be found in euthyroid patients with autonomous thyroid function (ophthalmic Graves' disease, autonomous nodules). It may be seen also as a compensatory phenomenon in patients with subclinical hypothyroidism and elevated TSH secretion (10).

In severe hypothyroidism, total T3 levels are usually low, but in moderate hypothyroidism they may be normal (11). In severe non-thyroidal illness or after surgery, T3 levels are often low, with elevated production of reverse T3 (4,12). T3 levels are generally higher in the newborn and infants and may be lower in older people (13).

Serum T3 values also vary with TBG concentration, being lower when TBG levels are low (as in liver disease, or congenital TBG deficiency) and higher during pregnancy or estrogen therapy (14,15).

The Eclectica assays for the Eclectica analyzers consists of:

  1. Eclectica Total T3 Reagent Kit: specific reagents of Eclectica Total T3 kit to execute the test and to verify the standardization.
  2. Eclectica Total T3 Calibration Kit: for standardization.
  3. Eclectica Immunoassay Common Reagents Kit: for general chemistry reaction.
  4. Eclectica Immunoassay Wash Solution: Wash Solution specific for Immunological reaction.
  5. Eclectica Systemic Wash Solution: Wash Solution of Eclectica system.

In the Eclectica Total T3 assay, a high affinity monoclonal antibody is used in an enzymatic immunoassay system which incorporates magnetic solid phase separation. All incubation times and reagent volumes are determined by an assay specific software protocol.

Fixed amounts of fluorescein-T3 derivative and anti-T3 monoclonal antibody conjugated to alkaline phosphatase are added to a sample, control or standard. 8 anilino-1-napthalene- sulfonate (ANS) is used to displace T3 from proteins to enable the measurement of total circulating T3. The reactants are incubated at 37°C. During the incubation fluorescein-T3 derivative and T3 present in the sample, control, or standard compete for binding to the anti-T3 monoclonal antibody-alkaline phosphatase conjugate.

The amount of fluorescein T3 derivative bound by the antibody is inversely related to the concentration of T3 in the sample.

At the end of the incubation period anti-fluorescein coupled to a magnetic solid phase is added in excess. This rapidly and specifically binds to the fluorescein-T3 derivative-antibody complex and is sedimented in a magnetic field.

After aspirating the liquid phase and washing the solid phase a substrate solution, phenolphthalein monophosphate, is added to the cartridge and the cartridge incubated at 37°C. The enzyme reaction is stopped by the addition of stop reagent and the intensity of the color developed is measured photometrically.

The intensity of the color developed is inversely proportional, within the working range of the assay, to the concentration of T3 in the sample. The concentration of T3 in a patient sample or control is then determined by interpolation from the stored standard curve.

Eclectica Total T3 Reagent Kit

Eclectica Total T3 Calibration Kit

Eclectica Immunoassay Common Reagents Kit

Eclectica Immunoassay Wash Solution

Eclectica Systemic Wash Solution

1700006 Eclectica Cleaning Kit

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