MicroELISA

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The EIAgen Helicobacter Pylori IgA Kit is an enzyme-linked immunosorbent assay method for determination of IgA-class antibodies to H. Pylori in human serum

In 1983, Warren and Marshall (1) identified Helicobacter pylori, a new gram-negative bacterial pathogen, in patients suffering from gastritis, and this finding led to studies on the relationship between bacterial infection and chronic gastric disease (2). The pathogen has been shown to be associated with peptic ulcer, with chronic type-B gastritis and with duodenitis. It has been demonstrated that in patients with gastritis, eradication of the bacteria led to healing of the anatomical lesion (4).

Diagnostic procedures for the detection of the organism generally involve invasive (gastroscopic) techniques for sample collection.

However, a specific immune response is seen in infected patients. The serological test thus represents a useful alternative to the invasive bioptic technique. IgG levels rise with infection and remain constantly high until the infection is eliminated. The efficacy of antimicrobial therapy can therefore be monitored via changes in specific IgG antibody. The determination of the IgA antibody levels is complementary to that of the IgG. As the IgA levels decrease more rapidly than the IgG in some patients undergoing treatment, this parameter can be useful in the follow-up of patients.

For the same reason, a low IgA level in IgG-positive patients may indicate a past infection. For unknown reasons, about 2% of serum samples are positive only for IgA (5,6).

The test is based on the ELISA technique (Enzyme linked Immunosorbent Assay) (5).

The antigen is bound to the solid phase. The specific immunoglobulins are bound to the antigen through incubation with dilute human serum.

After washings to eliminate the proteins which have not reacted, incubation is performed with the conjugate, composed of anti-human IgA monoclonal antibodies labelled with peroxidase.

The unbound conjugate is eliminated, and the peroxidase substrate added.

The density of the colour which develops is proportional to the concentration of specific antibodies present in the serum sample. When the enzymatic reaction is interrupted by the addition of a sulfuric acid solution, the resulting yellow colour can be easily read on an ELISA microplate reader.

The kit contains reagents for 96 tests (code 081047).

Bring reagents to room temperature before use.