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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Serum||EIAgen FSH Kit||Tests per Package: 96|
|EIAgen||EIAgen FSH is a direct solid phase enzyme for the quantitative determination of Follicle-Stimulating Hormon (FSH) in human serum.||Code: LI4013F1||Package: 1 Microplate|
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EIAgen FSH is a direct solid phase enzyme for the quantitative determination of Follicle-Stimulating Hormon (FSH) in human serum.
Follicle Stimulating hormone (FSH) is a glycoprotein consisting of two subunits with an approximate molecular mass of 35,500 daltons. The α-subunit is similar to other pituitary hormones [luteinizing stimulating hormone (LH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (CG)] while the b-subunit is unique.
The b- subunit confers the biological activity to the molecule. Stimulation by gonadotropin-releasing hormone (GnRH) causes release of FSH, as well as LH, from the pituitary and is transported by the blood to their sites of action, the testes or ovary.
In men, FSH acts on the Sertoli cells of the testis, stimulating the synthesis of inhibin, which appears to specifically inhibit further FSH secretion, and androgen-binding protein. Thus, it indirectly supports spermatogenesis.
In women, FSH acts on the granulosa cells of the ovary, stimulating steroidogensis. All ovulatory menstrual cycles have a characteristic pattern of FSH, as well as LH, secretion. The menstrual cycle is divided into a follicular phase and a luteal phase by the midcycle surge of the gonadotropins (LH and FSH). As the follicular phase progresses, FSH concentration decreases. Near the time ovulation occur, about midcycle, FSH peaks (lesser in magnitude than LH) to its highest level.
The clinical usefulness of the measurement of Follicle Stimulating hormone (FSH) in ascertaining the homeostasis of fertility regulation via the hypothalamic - pituitary - gonadal axis has been well established (1,2).
In this method, calibrators, patient specimens and/or controls (containing the native FSH antigen) are first added to streptavidin coated wells. Biotinylated monoclonal and enzyme labeled antibodies are then added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of FSH.
Reaction between the various FSH antibodies and native FSH occurs in the microwells without competition or steric hindrance, forming a soluble sandwich complex.
Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody.
After equilibrium is attained, the antibody-bound fraction is separated from unbound antigen by decantation or aspiration. The enzyme activity in the antibody-bound fraction is directly proportional to the native antigen concentration.
The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color.
By utilizing several different Calibrators of known antigen values, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI4013F1).
Bring reagents to room temperature before use.
Wash Buffer Conc. 50X
Number of tests