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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Serum||EIAgen Free T4 Kit||Tests per Package: 96|
|EIAgen||EIAgen Free T4 is a direct solid phase enzyme immunoassay for the quantitative determination of free Thyroxine (fT4) in human serum.||Code: LI4021||Package: 1 Microplate|
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EIAgen Free T4 is a direct solid phase enzyme immunoassay for the quantitative determination of free Thyroxine (fT4) in human serum.
Thyroxine, the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. The main carrier is thyroxine binding globulin (TBG). However, only the free (unbound) portion of thyroxine is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total thyroxine level changes so that the free thyroxine concentration remains constant. Thus, measurements of free thyroxine concentrations correlate better with clinical status than total thyroxine levels. The increase in total thyroxine associated with pregnancy, oral contraceptives and estrogen therapy occasionally result in total T4 levels over the limits of normal while the free thyroxine concentration remains in the normal reference range. Masking of abnormal thyroid function can also occur in both hyper and hypothyroid conditions by alterations in the TBG concentration. The total T4 can be elevated or lowered by TBG changes such that the normal reference levels result.
The free thyroxine concentration can help in uncovering the patient’s actual clinical status.
Competitive Enzyme Immunoassay – Analog Method for Free T4
The essential reagents required for this solid phase enzyme immunoassay include antibody anti-T4 coated on microwells, enzyme -antigen conjugate and native T4 antigen.
Upon mixing, in the coated microwells, the enzyme-antigen conjugate and a serum containing the native free T4 antigen, a competition reaction results between the native free T4 antigen and the enzyme-antigen conjugate for a limited number of insolubulized binding sites.
After equilibrium is attained, the antibody-bound fraction is separated from unbound antigen by decantation or aspiration. The enzyme activity in the antibody-bound fraction is inversely proportional to the native free antigen concentration.
The activity of the enzyme present on the surface of well is quantitated by reaction with a substrate to produce colour.
By utilizing several different calibrators of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI4021).
Bring reagents to room temperature before use.
Wash Buffer Conc. 50X
Number of tests