MicroELISA

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EIAgen CEA kit is a direct solid phase enzyme immunoassay for quantitative determination of Carcinoembryonic Antigen (CEA) in human serum.

Carcinoembryonic antigen (CEA) is a glycoprotein with a molecular weight of 180 kDA. CEA is the first of the so-called carcinoembryonic proteins that was discovered in 1965 by Gold and Freeman (1).

CEA is the most widely used marker for gastrointestinal cancer. Although CEA is primarily associated with colorectal cancers, other malignancies that can cause elevated levels of CEA include breast, lung, stomach, pancreas, ovary and other organs. Benign conditions that cause significantly higher than normal levels include inflammation of lung and gastrointestinal (GI) tract and benign liver cancer (2,3).

Heavy Smokers, as a group, have higher than normal baseline concentration of CEA.

Immunoenzymometric assay:

In this method, calibrators, patient specimens and/or controls (containing the native CEA antigen) are first added to streptavidin coated wells. Biotinylated monoclonal and enzyme labeled antibodies are then added and the reactants mixed: these antibodies have high affinity and specificity and are directed against distinct and different epitopes of CEA.

Reaction between the various CEA antibodies and native CEA occurs in the microwells without competition or steric hindrance, forming a soluble sandwich complex.

Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody.

After equilibrium is attained, the antibody-bound fraction is separated from unbound antigen by decantation or aspiration. The enzyme activity in the antibody-bound fraction is directly proportional to the native antigen concentration. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce colour. By utilizing several different calibrators of known antigen values, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.

The kit contains reagents for 96 tests (code LI4022F1).

Bring reagents to room temperature before use.