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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Serum||EIAgen Folate Kit||Tests per Package: 96|
|EIAgen||The EIAgen Folate Kit has been designed for the quantitative determination of Folate Concentration in Human Serum.||Code: LI5001||Package: 1 Microplate|
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The EIAgen Folate Kit has been designed for the quantitative determination of Folate Concentration in Human Serum.
Folate supplementation has escalated over recent years with the knowledge of its many benefits. As one of the B vitamins, folate, or Vitamin B9, is involved in many bodily functions and deficiency can cause disease in not only the elderly, but infants too. Folate deficiency is associated with megaloblastic anemia, neural tube defects, and cardiovascular dieases.1,2,3
Folate plays an important role in brain development and therefore is vital during growth. The most common defects resultant from folate deficiencies are neural tube defects. With a vital role in nucleic acid synthesis, folate has been found to be beneficial as supplementation during pregnancy and other times of rapid tissue growth. Folate also plays a vital role in maintaining proper balance of homocysteine, a contributing factor in occurrences of occlusive vascular diseases and stroke. Individuals with susceptibility to heart disease and several forms of cancer may also benefit from supplementation. 1,2,4
Major sources of folate include green leafy vegetables, legumes, beans and fortified cereals. Foods fortified with folate are actually fortified with folic acid because of the higher bioavailability for absorption by the body. In circulation, folate is present in several different forms, some of which are more stable than others. Folic acid and N-methyltetrahydofolate are two common forms, the latter being more stable and found in higher concentrations in serum. Due to the stability of the molecule, methytetrahydrofolate is very often used as the form focused on during methods of analysis.4,5
Folate binding proteins are responsible for folate metabolism. Two types exist in circulation: one type aids in binding to the cell surface and the other soluble form exists in circulation. These folate binding proteins also have the capability of binding several different folate derivatives including folic acid and N-methytetrahydrofolate. The interaction between folic acid and folate binding protein is greater than methyltetrahydrofolate. Current assays on the market require an extraction step to release the folate derivatives from the folate binding protein.5,6
In the past, folate has been quantified in samples using such methods as microbiological assays, bio-specific procedures and HPLC-MS techniques. Overall, this rapid rise in knowledge of folate, its importance, and subsequently folate supplementation has caused a higher demand for improved testing methods.4
Competitive Binding Protein Assay (TYPE 8):
The essential reagents required for a competitive binding assay include specific binding protein, enzyme-antigen conjugate and native antigen. Upon mixing enzyme-antigen conjugate, biotinylated binding protein and a serum containing the native antigen, a competition reaction results between the native antigen and the enzyme-antigen conjugate for a limited number of binding sites.
A simultaneous reaction between the biotin attached to the binding protein and the streptavidin immobilized on the microwell occurs. This effects the separation of the binding protein enzyme bound fraction after decantation or aspiration.
The enzyme activity in the protein binding protein bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI5001).
Bring reagents to room temperature before use.
Wash Buffer Conc. 50X
Number of tests