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|Method & Line||Sample & Target||Product||Package Info|
|MicroELISA||Serum||EIAgen Vitamin B-12 Kit||Tests per Package: 96|
|EIAgen||The EIAgen Vitamin B-12 Kit has been designed for the quantitative determination of Vitamin B-12 Concentration in Human Serum.||Code: LI5000||Package: 1 Microplate|
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The EIAgen Vitamin B-12 Kit has been designed for the quantitative determination of Vitamin B-12 Concentration in Human Serum.
Vitamin B-12 is one of the nine water soluble vitamins important for healthy body functioning. The most important roles Vitamin B-12 plays in the human body are in the formation of red blood cells and the formation of the myelin sheath around the nerves. Since the effects are seen in body systems with a large range of function, the symptoms of Vitamin B-12 deficiency can sometimes be very ambiguous.A deficiency may also take from months to years to manifest depending on the cause and severity.,2,3
Two of the most common causes of Vitamin B-12 deficiency are diet and age. Because most sources of dietary Vitamin B-12 come from animals, vegans who do not efficiently supplement their diet are at risk. The elderly community is also at high risk because of their diet, as well as the less efficient functioning of their digestive system.1,3,4
Intake of Vitamin B-12 starts by ingestion and then digestion by saliva. Once reaching the gut, Vitamin B-12 bound to proteins in food are released by the acids present. The B12 can then bind the Intrinsic factor. Once bound to IF, Vitamin B-12 is stable enough to travel into the intestines where it can be absorbed into your body through of its association with IF.1,5,6,7
Two very useful tests to distinguish between Vitamin B-12 deficiency and folate deficiency are methylmalonyl CoA (MMA) and homocysteine (hcy). Both deficiencies are represented by similar symptoms; however, even though both show increased levels of homocysteine, only Vitamin B-12 deficiency causes an increase in methylmalonyl CoA.The increase in levels of methylmalonyl CoA and homocysteine is thought to be the root cause of any symptoms that accompany a Vitamin B-12 deficiency. High levels of these two analytes in the blood stream causes increased oxidative stress to cells therefore causing increased apoptosis. In turn, vascular disease results in the form of atherosclerosis, coronary heart disease and/or neurodegeneration (ex. Parkinson’s Disease).1,8,9.
Delayed Competitive Enzyme Immunoassay (TYPE 9):
The essential reagents required for an enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen.
Upon mixing the biotinylated antibody with a serum containing the antigen, a reaction results between the antigen and the antibody.
After a short incubation, the enzyme conjugate is added (this delayed addition permits an increase in sensitivity for low concentration samples). Upon the addition of the enzyme conjugate, competition reaction results between the enzyme analog and the antigen in the sample for a limited number of antibody binging sites (not consumed in the first incubation).
A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration.
The enzyme activity in the antibody bound fraction is inversely proportional to the native antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
The kit contains reagents for 96 tests (code LI5000).
Bring reagents to room temperature before use.
Vitamin B-12 Biotin
Wash Buffer Conc. 50X
Number of tests