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Molecular Diagnostic Test Product Information & Purchase Order

Line Method & Sample Product Package Info
MOLgen Clinical Specimens MOLgen DNA Candida Albicans S1 Kit Tests per Package: 96
Candidiasis “MOLgen DNA Candida albicans S1 Kit” is an assay kit for the detection of Candida albicans DNAby Real-Time PCR method Code: ME146980 Package Format: STR
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  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

  • Stock:
  • Available
    *Usually shipping within 5 business days
  • Min Order:
  • 5 Kits
  • Shipping:
  • Not Included

For Quantity Orders: Request a Quote

Please pay attention to the revision of the document that must be the same as the revision reported in the box label.
In case of discrepancy please contact our Customer Care e-mail: info@adaltis.net.

* Other document related to the product available at Documentation Centre and it is accessible for Adaltis distributors/partners after registration only.

“MOLgen DNA Candida albicans S1 Kit” is an assay kit for the detection of Candida albicans DNAby Real-Time PCR method.

“MOLgen DNA Candida albicans S1 Kit” is intended for the detection of Candida albicans DNA, extracted from clinical specimens (epithelial cells swabs, semen, prostatic fluid, urine), using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product.

Set 1 is intended for use with block-type PCR cyclers: iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia).

The extraction of DNA can be performed using the“Molgen Universal Extraction Kit”.

When using NA extraction kits of other manufacturers it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis.

Set 1 contains reagents requiredfor 96 tests, including control samples.

Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis.

Threshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold and the fluorescence signal rises significantly above the background. Increased signal is due to the use of a DNA hybridization probe that is specific for the given DNA sequence: it binds to one of the DNA strands in the course of reaction and provides additional specificity of the method. A DNA probe consists of a fluorescence dye at the 5’-end and a fluorescence quencher at the 3’-end that significantly reduces the fluorescence intensity. During the polymerase synthesis of the complementary strand, the probe is cleaved from the 5’-end due to the 5’-3’ nuclease activity of Taq DNA polymerase, the quencher and the dye become separated, thus increasing the fluorescence signal due to accumulation of the reaction product. The detected fluorescence intensity depends on initial quantity of pathogens DNA template in the sample.

The use of Internal Control sample (IC) prevents generation of false negative results associated with possible loss of DNA template during specimen preparation. IC indicates if PCR inhibitors occur in the reaction mixture. IC should be added to each sample (including control samples) prior to the DNA extraction procedure. Amplification and detection of IC influence neither sensitivity nor specificity of the target DNA PCR.

Note: Internal Control sample is added to the specimen during NA extraction step and is used throughout the whole process of NA extraction, amplification, detection.

Reagent

Content

Universal Positive Control Sample (PC)

1 tube, 1.0 mL

Ready Master Mix for PCR (RMM)

(lyophilized)

96 tubes

PCR optical-quality film

1.5 sheet

Number of tests

96

Code

ME146980

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