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The Most Stabilized & Most Sensitive Single Solution substrate for ELISA assays
TMB (3,3´,5,5´-Tetramethylbenzidine) is a chromogen that yields a blue colour when oxidized, typically as a result of oxygen radicals produced by the hydrolysis of hydrogen peroxide by HRP.
For kinetic or non-stopped ELISA assays, the TMB chromogen has maximal absorbance at 370 nm and 650 nm. The colour then changes to yellow with the addition of sulphuric or phosphoric acid with maximum absorbance at 450 nm.
|10 litres||Sufficient for about 1000 microplates|
|20 litres||Sufficient for about 2000 microplates|
|50 litres||Sufficient for about 5000 microplates|
|100-200 ml.||Available on request|
The TMB solution is added at the end of ELISA protocol to each microplate well:
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