IVD SYSTEMS & AUTOMATED CLINICAL DIAGNOSTIC ANALYSERS
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Hybridomas & Monoclonal Antibody Production

Reliability & Experience

Hybridomas (Cell Lines) & Monoclonal Antibody Production

Adaltis  can produce purified  and  not purified  antibody  by using  its  cell lines. 

Antibodies   can  be purified   on request  by: HPLC , FPLC , affinity chromatography  and gel  filtration.

For production service information please contact: info@adaltis.net

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MONOCLONAL  ANTIBODIES  PRODUCTION  

Method  :  HYBRIDOMA  TECHNOLOGY 

  • Hybridoma  Technology developed by Kohler and Milstein has been widely used for the  production of Monoclonal Antibodies.
  • Monoclonal Antibodies (MAbs) are antibodies that arise from  a single clone of cells.
  • They are homogenous in a plasma cell tumour (Myeloma).
  • B cells can produce antibodies of single specificity.
  • Myeloma cells or tumor cells are capable of continuous division forming large number of cells .
  • The fusion of two types of cells form cells called hybridomas.
  • Hybrid cells will be having the property of B cells antibody production and tumour cells  ability of continuous division.
  • Hybrid cell ensures antibody production  continuously  in dividing cells.
  • The mixture of cells are cultured under  conditions which permit  growth of only hybridoma cells .
  • Each hybridoma cell will produce a single type of antibody against a single epitope.
  • The hybridoma cells producing the desired monoclonal antibody are the  cultured
  • Monoclonal antibodies are isolated and purified.

Hybridoma  Technology

Monoclonal antibodies

Monoclonal antibodies

Hybridomas (Cell Lines) & Monoclonal Antibody Production List

Product Name Application Code Affinity Costant
AFP. 01 Alfa-Fetoprotein AFP 0184 9,60 x 1010 l/mol
AFP. 02 Alfa-Fetoprotein AFP 0280 4,20 x 1010 l/mol
CEA . 01 Carcinoembryonic Antigen CEA 0184 1,48 x 1010 l/mol
CEA . 02 Carcinoembryonic Antigen CEA 0280 1,88 x 1010 l/mol
FER . 15 Ferritin FER 1580 1,30 x 109 l/mol
FSH . 08 FSH FSH 0882 3,80 x 1011 l/mol
FSH . 13 FSH FSH 1380 4,30 x 109 l/mol
HCG . 01 HCG HCG 0184 Not available
HCG . 02 HCG HCG 0284 2,00 x 1011 l/mol
HCG . 03 HCG HCG 0380 6,00 x 1010 l/mol
HLH . 01 LH HLH 0184 6,50 x 1010 l/mol
HLH.02 LH HLH 0280 1,60 x 1010 l/mol
HPRL . 04 Prolactin HPRL 0484 1,80 x 1010 l/mol
INS . 01 Insulin INS 0181 11,00 x 108 l/mol
INS . 02 Insulin INS 0284 4,00 x 109 l/mol
PRG . 02 Progesteron PRG 0284 Not available
TSH . 01 TSH TSH 0184 6,00 x 1010 l/mol
TSH . 03 TSH TSH 0380 1,00 x 1010 l/mol
TSH . 05 TSH TSH 0582 1,00 x 1011 l/mol
T3T . 01 Total T3 T3T 0184 2,45 x 1010 l/mol
T4T . 02 Total T4 T4T 0284 2,10 x 109 l/mol
IgE .01D6 Allergy Line IgE .01D6 Not determined
IgE .02 Allergy Line IgE .02 Not determined
R22T Rubella R22T Not determined
Anti-S2 HBsAg S2B3 Not determined
Anti-S3 HBsAg S3DT Not determined
IgM1 ToRCH IgM1 Not determined
TO5T Toxoplasma TO5T Not determined
Core 1/39 Anti-Core Core 1/39 Not determined