MicroELISA

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Enzyme ImmunoAssay (ELISA) for the quantitative/qualitative determination of IgG antibodies to Cytomegalovirus in plasma and sera. For Research Use Only.

Cytomegalovirus or CMV is an ubiquitous human pathogen, whose infection is particular prevalent among children and young adults. Infections by CMV continue to be an important health problem in certain patient populations, such as newborns, graft recipients of solid organs or bone marrow and AIDS patients. In these groups CMV is a major cause of morbidity and mortality.

The detection of virus-specific IgG and IgM antibodies is of great value in the diagnosis of acute/primary virus infections or reactivation of a latent one, in the absence of typical clinical symptoms. Asymptomatic infections usually happen for CMV in apparently healthy individuals, during pregnancy and several diseases as a coinfective agent.

Microplates are coated with native Cytomegalovirus antigens, highly purified by sucrose gradient centrifugation and inactivated.

The solid phase is first treated with the diluted sample and IgG to Cytomegalovirus are captured, if present, by the antigens.

After washing out all the other components of the sample, in the 2^nd incubation bound anti Cytomegalovirus IgG are detected by the addition of polyclonal specific anti hIgG antibodies, labelled with peroxidase (HRP).

The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti Cytomegalovirus IgG antibodies present in the sample. A Calibration Curve, calibrated against the Anti-CMV IgG Paul Ehrlich Institute (PEI) Reference material  containing 300 AU/mL (arbitrary unit), makes possible a quantitative determination of the IgG antibody in the patient.

Each kit contains sufficient reagents to perform 96 tests (code 091004).