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|Method & Line
|Sample & Target
|EIAgen Toxoplasma IgG Kit
|Tests per Package: 96
|Enzyme ImmunoAssay (ELISA) kit for the quantitative/qualitative determination of IgG antibodies to Toxoplasma gondii in human serum or plasma. For Research Use Only.
|Package: 1 Microplate
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Enzyme ImmunoAssay (ELISA) kit for the quantitative/qualitative determination of IgG antibodies to Toxoplasma gondii in human serum or plasma. For Research Use Only.
Toxoplasma gondii (T.gondii) is an obligate intracellular protozoan parasite that is probably capable of infecting all species of mammals, including man. The separately detection of specific IgG and IgM antibodies to T.gondii is particularly helpful for the diagnosis of acute infections in “risk” individuals, in association with AIDS, organ transplantation and pregnancy. As most of T.gondii infections are mild or asymptomatic in otherwise healthy individuals, the detection of T.gondii specific IgM antibodies, in absence of detectable specific IgG, has become important for the monitoring of acute infections in pregnant women, as the parasite can lead to severe birth defects. Moreover, as T.gondii infections are most severe in immunocompromised patients, where the disease can be fatal, acute infections due to this parasite have to be distinguished from other disorders. In addition, recently developed IgM assays by the capture method provide the clinician with a helpful and reliable test, not affected by the rheumatoid factor as it happens to be in classic sandwich tests.
Microplates wells are coated with native T. gondii antigens, highly purified by sucrose gradient centrifugation and inactivated.
The diluted sample is incubated into the coated wells and, if present, the specific antibodies to T. gondii are bound, , by the coated antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti Toxoplasma gondii IgG are detected by a of polyclonal specific anti human IgG antibodies, labelled with peroxidase (HRP).
Then, the bound enzyme conjugate on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti Toxoplasma gondii IgG antibodies present in the sample. A Calibration Curve with calibrators made traceable to the World Health Organization (WHO).International Standard code 01/600, makes it possible a quantitative determination of the IgG antibody of the patient.
Each kit contains sufficient reagents to perform 96 tests (code 091000).
Calibrator 1 / 2 / 6
Calibrator 3 / 4 / 5
Wash Buffer Concentrate 20x
Plate seal foils
Number of tests